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Comparative analysis of growth performance and liver transcriptome response of juvenile Ancherythroculter nigrocauda fed diets with different protein levels

文献类型： 外文期刊

作者： Lu, Xing 1 ; Wen, Hua 2 ; Li, Qing 1 ; Wang, Guiying 1 ; Li, Pei 1 ; Chen, Jim 1 ; Sun, Yanhong 1 ; Yang, Changgeng 2 ; Wu, 1 ;

作者机构： 1.Wuhan Acad Agr Sci, Fisheries Res Inst, Wuhan 430207, Hubei, Peoples R China

2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Hubei, Peoples R China

3.Wuhan Xianfeng Aquaculture Technol Co Ltd, Wuhan 430207, Hubei, Peoples R China

关键词： Ancherythroculter nigrocauda; Dietary protein level; Growth performance; Liver transcriptome; Gene expression

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.674； 五年影响因子：2.941 ）

ISSN： 1744-117X

年卷期： 2019 年 31 卷

页码：

收录情况： SCI

摘要： This study aimed at investigating the effects of dietary protein levels on the growth and liver transcriptome in juvenile Ancherythroculter nigrocauda. Six semi-purified diets were formulated containing 25 (control), 30, 35, 40, 45, and 50% protein. Each diet was fed to three groups of 35 fish (mean initial weight: 5.86 +/- 0.10 g) for 56 days. The rate of weight gain and specific growth rate increased with dietary protein levels from 25% to 40%, but remained unchanged when fed with 45 or 50% dietary protein. The feed conversion ratio was significantly influenced by the dietary protein levels, being the lowest in fish fed 40% protein Illumina RNA-seq analysis was performed to investigate liver gene expression changes under different dietary protein treatments. A total of 367.78 million clean reads were obtained from the six libraries. Compared with 25% protein treatment library, there were 734, 1946, 1755, 2726, and 1523 upregulated genes, and 407, 1882, 1865, 2216 and 1624 down-regulated genes in the 30, 35, 40, 45, and 50% protein treatment libraries, respectively. Trend analysis of these differentially expressed genes (DEGs) identified six statistically significant trends. A series of DEGs that related to protein metabolism, growth and development, lipid metabolism and immune and stress response were identified. Moreover, gene ontology enrichment analysis of the DEGs demonstrated that cellular process, single-organism process, metabolic process and biological regulation were the most highly overrepresented biological processes. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that protein processing in endoplasmic reticulum, PPAR signaling pathway, complement and coagulation cascades, and cytochrome P450 (CYP450s) were significantly enriched in the dietary protein treatment groups. Furthermore, qPCR results showed excellent agreement on those of RNA-seq for both up- and down-regulated genes (including fasn, acca, SCD, CPT-I, igf1, ST, AST, trdmt1, hsp70, cyp450, MHC-II, C4, tgf beta, ube4b, apoE and abcb7). Thus, our results provide the baseline information for the feed formulation and nutritional research for A. nigrocauda.

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