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Label-Free Fluorescent Aptasensor for Ochratoxin-A Detection Based on CdTe Quantum Dots and (N-Methyl-4-pyridyl) Porphyrin

文献类型： 外文期刊

作者： Liu, Li 1 ; Tanveer, Zafar Iqbal 1 ; Jiang, Keqiu 1 ; Huang, Qingwen 1 ; Zhang, Jinghui 1 ; Wu, Yongjiang 1 ; Han, Zhe 1 ;

作者机构： 1.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China

2.Shanghai Acad Agr Sci, Lab Qual & Safety Risk Assessment Agroprod Shangh, Shanghai Key Lab Protected Hort Technol, Minist Agr,Inst Agrofood Stand & Testing Technol, Shanghai 201403, Peoples R China

关键词： ochratoxin A; porphyrin; quantum dots; G-quadruplex aptamer

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN：

年卷期： 2019 年 11 卷 8 期

页码：

收录情况： SCI

摘要： With the widespread contamination of ochratoxin A (OTA), it is of significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing the interaction between OTA-triggered antiparallel G-quadruplex and (N-methyl-4-pyridy) porphyrin (TMPyP) for the rapid and sensitive determination of OTA was established. The fluorescence of CdTe quantum dots (QDs) could be quenched by TMPyP. In the presence of analyte (OTA), the aptamer could recognize OTA and transform from a random coil to the antiparallel G-quadruplex. The interaction between G-quadruplex and TMPyP could release CdTe QDs from TMPyP, and thus recover the fluorescence of CdTe QDs. Under optimized conditions, the detection limit of the designed aptasensor was 0.16 ng mL(-1), with a linear range of 0.2 to 20 ng mL(-1). Furthermore, this aptasensor showed high selectivity toward OTA against other structural analogs and other mycotoxins, and was successfully applied in Astragalus membranaceus samples. The presented aptasensor for OTA detection could be a promising tool for the field monitoring of food and TCM.

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