Label-Free Fluorescent Aptasensor for Ochratoxin-A Detection Based on CdTe Quantum Dots and (N-Methyl-4-pyridyl) Porphyrin
文献类型： 外文期刊
作者： Liu, Li 1 ; Tanveer, Zafar Iqbal 1 ; Jiang, Keqiu 1 ; Huang, Qingwen 1 ; Zhang, Jinghui 1 ; Wu, Yongjiang 1 ; Han, Zhe 1 ;
作者机构： 1.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China
2.Shanghai Acad Agr Sci, Lab Qual & Safety Risk Assessment Agroprod Shangh, Shanghai Key Lab Protected Hort Technol, Minist Agr,Inst Agrofood Stand & Testing Technol, Shanghai 201403, Peoples R China
关键词： ochratoxin A; porphyrin; quantum dots; G-quadruplex aptamer
期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）
ISSN：
年卷期： 2019 年 11 卷 8 期
页码：
收录情况： SCI
摘要： With the widespread contamination of ochratoxin A (OTA), it is of significant importance for detecting OTA in foods and traditional Chinese medicine (TCM). In this study, a novel label-free fluorescent aptasensor utilizing the interaction between OTA-triggered antiparallel G-quadruplex and (N-methyl-4-pyridy) porphyrin (TMPyP) for the rapid and sensitive determination of OTA was established. The fluorescence of CdTe quantum dots (QDs) could be quenched by TMPyP. In the presence of analyte (OTA), the aptamer could recognize OTA and transform from a random coil to the antiparallel G-quadruplex. The interaction between G-quadruplex and TMPyP could release CdTe QDs from TMPyP, and thus recover the fluorescence of CdTe QDs. Under optimized conditions, the detection limit of the designed aptasensor was 0.16 ng mL(-1), with a linear range of 0.2 to 20 ng mL(-1). Furthermore, this aptasensor showed high selectivity toward OTA against other structural analogs and other mycotoxins, and was successfully applied in Astragalus membranaceus samples. The presented aptasensor for OTA detection could be a promising tool for the field monitoring of food and TCM.
- 相关文献
作者其他论文 更多>>
-
Preparation and characterization of the aflatoxin B1 purity certified reference material (GBW (E) 100599)
作者：Guo, Wenbo;Li, Zenghe;Guo, Wenbo;Meng, Jiajia;Wang, Xinyi;Li, Jianchi;Niu, Xueke;Zhao, Zhihui;Han, Zheng;Guo, Wenbo;Niu, Xueke
关键词：aflatoxin B1; Mass balance method; Quantitative nuclear magnetic resonance; Uncertaintity; Purity certified reference material
-
Perspective Accelerated photodegradation of T-2 toxin over magnetic recyclable ZnO/ CaFe 2 O 4 nanocomposite with a p-n based Z-scheme heterojunction architecture
作者：Huang, Qingwen;Lou, Xiuping;Nie, Dongxia;Zhao, Zhihui;Fan, Kai;Guo, Wenbo;Meng, Jiajia;Han, Zheng;Lou, Xiuping;Han, Zheng;Liu, Zheyuan
关键词：T-2 toxin; Photocatalytic degradation; Z-scheme; p-n heterojunction
-
Molecularly imprinted polymer-based electrochemical sensor for rapid detection of masked deoxynivalenol with Mn-doped CeO2 nanozyme as signal amplifier
作者：Nie, Dongxia;Zhu, Xueting;Liu, Minghui;Cheng, Meng;Fan, Kai;Zhao, Zhihui;Huang, Qingwen;Han, Zheng;Zhang, Xiaolin
关键词：Deoxynivalenol-3-glucoside; Molecularly imprinted polymer; Electrochemical sensor; Mn-CeO 2; Nanozyme
-
Antifungal Activity and Action Mechanisms of 2,4-Di-tert-butylphenol against Ustilaginoidea virens
作者：Fan, Kai;Yu, Yinan;Hu, Zheng;Qian, Shen'an;Zhao, Zhihui;Meng, Jiajia;Zheng, Simin;Huang, Qingwen;Zhang, Zhiqi;Nie, Dongxia;Han, Zheng;Yu, Yinan;Zheng, Simin;Han, Zheng
关键词：Ustilaginoidea virens; 2,4-di-tert-butylphenol; antifungal activity; inhibitory mechanism
-
Reduced graphene oxide-zinc iron oxide nanomaterial as selective dispersive solid-phase extraction sorbent for extraction and enrichment of aflatoxins from milk combined with UHPLC-MS/MS analysis
作者：Tanveer, Zafar Iqbal;Xu, Tengfei;Chen, Yong;Liu, Xuesong;Wu, Yongjiang;Huang, Qingwen;Han, Zheng
关键词：Aflatoxins; Milk; Dispersive solid phase extraction; Reduced graphene oxide; UHPLC-MS/MS
-
Kinetics and Distribution of Zearalenone-14-Glucoside and Its Metabolite Zearalenone in Rat, Determined by a Reliable HPLC-MS/MS Method
作者：Cai, Yaling;Li, Zhizhong;Cai, Yaling;Zhang, Zhiqi;Dong, Fang;Ma, Zefeng;Fan, Kai;Han, Zheng;Zhao, Zhihui
关键词：zearalenone-14-glucoside; zearalenone; HPLC-MS; MS; rat plasma; urine; tissues
-
Survey and toxigenic abilities of Aspergillus, Fusarium, and Alternaria fungi from wheat and paddy grains in Shanghai, China
作者：Meng, Jiajia;Huang, Qingwen;Fan, Kai;Zhang, Jingya;Zhu, Xueting;Chen, Xinyue;Nie, Dongxia;Zhao, Zhihui;Han, Zheng;Li, Ruijiao;Han, Zheng;Guo, Dehua;Wang, Min;Cao, Chen
关键词：mycotoxins; toxigenic ability; Fusarium spp; Aspergillus spp; Alternaria spp
