Purification and Identification of Antioxidant Peptides from Schizochytrium Limacinum Hydrolysates by Consecutive Chromatography and Electrospray Ionization-Mass Spectrometry
文献类型： 外文期刊
作者： Hu, Xiao 1 ; Yang, Xianqing 1 ; Wu, Qiong 1 ; Li, Laihao 1 ; Wu, Yanyan 1 ; Chen, Shengjun 1 ; Li, Ruijie 1 ; Ren, Jiaoya 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Aquat Prod Proc, Guangzhou 510300, Guangdong, Peoples R China
2.Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
3.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Shandong, Peoples R China
4.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China
关键词： Schizochytrium limacinum hydrolysates; antioxidant peptides; radical scavenging activity; reducing power; electrospray ionization-mass spectrometry
期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）
ISSN：
年卷期： 2019 年 24 卷 16 期
页码：
收录情况： SCI
摘要： Schizochytrium limacinum residue was hydrolyzed with various proteases (papain, trypsin, Flavourzyme, Protamex, and Alcalase 2.4L) to obtain antioxidative peptides. The results showed that the S. limacinum hydrolysates (SLHs) prepared with compound proteases (Protamex and Alcalase 2.4L) had the highest antioxidant activity, which was measured using methods such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability (IC50 = 1.28 mg/mL), hydroxyl radical scavenging ability (IC50 = 1.66 mg/mL), and reducing power (1.42 at 5.0 mg/mL). The hydrolysates were isolated and purified by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). Through analysis of electrospray ionization-mass spectrometer (ESI-MS/MS), the purified antioxidant peptide was identified as Pro-Tyr-Lys (406 Da). Finally, the identified peptide was synthesized for evaluating its antioxidant activity. The center dot OH scavenging ability and reducing power of Pro-Tyr-Lys were comparable to those of reduced L-glutathione (GSH). These results demonstrated that the antioxidant peptides from SLHs could potentially be used as effective antioxidants.
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