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MHC Class IIB gene polymorphisms associated with resistance/susceptibility to Streptococcus agalactiae in Nile tilapia Oreochromis niloticus

文献类型： 外文期刊

作者： Gao, Feng-Ying 1 ; Zhang, Dong 1 ; Lu, Mai-Xin 1 ; Cao, Jian-Meng 1 ; Liu, Zhi-Gang 1 ; Ke, Xiao-Li 1 ; Wang, Miao 1 ; Zh 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

2.Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China

3.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

关键词： Nile tilapia; MHC Class IIB; Streptococcus agalactiae; Disease resistance

期刊名称：DISEASES OF AQUATIC ORGANISMS （ 影响因子：1.802； 五年影响因子：2.148 ）

ISSN： 0177-5103

年卷期： 2019 年 133 卷 3 期

页码：

收录情况： SCI

摘要： Genetic variation in the major histocompatibility complex (MHC) Class IIB was tested in Nile tilapia Oreochromis niloticus, and the association between the MHC IIB alleles and disease resistance was also studied. F-3 fry offspring (n = 1200) from 12 full-sib families were challenged with Streptococcus agalactiae, which caused significantly different mortalities in different Nile tilapia families (11.00-81.10%). Twenty fry (F-1) from each of the 12 families were selected to study the polymorphisms of the MHC Class IIB gene using PCR followed by cloning and sequencing methods. The results showed that the size of the amplified fragment was 770-797 bp. Thirty-seven sequences from 240 individuals revealed 22 different alleles, which belonged to 9 major allele types. Up to 63.58% of nucleotide positions were variable, while the proportion of the amino acid variable positions was up to 68.73%. According to the survival rate of offspring (F-3) from 12 full-sib families, we deduced that the alleles Orni-DAB*0107, Orni-DAB*0201 and Orni-DAB*0302 were highly associated with resistance to S. agalactiae, while the allele Orni-DAB*0701 was associated with susceptibility to S. agalactiae. In addition, our previous study found that the allele Orni-DAB*0201 was more frequently distributed in the disease-resistant groups. Therefore, the allele Orni-DAB*0201 could be used as an S. agalactiae resistance-related MHC marker in molecular marker-assisted selective breeding programs for S. agalactiae-resistant Nile tilapia.

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