文献类型： 外文期刊

作者： Xu, Yan-mei ¹ ; Xiao, Xian-mei ¹ ; Zeng, Ze-xiang ¹ ; Tan, Xiao-li ¹ ; Liu, Zong-li ¹ ; Chen, Jian-wen ² ; Su, Xin-guo ³ ;

作者机构： 1.South China Agr Univ, Coll Hort, Engn Res Ctr Southern Hort Prod Preservat,State K, Minist Educ,Guangdong Prov Key Lab Postharvest Sc, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Coll Agr, Minist Agr, Sci Observing & Expt Stn Crop Cultivat South Chin, Guangzhou 510642, Guangdong, Peoples R China

3.Guangdong Food & Drug Vocat Coll, Dept Food Sci, Guangzhou 510520, Guangdong, Peoples R China

关键词： Chinese flowering cabbage; leaf senescence; JA; transcriptional activation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1661-6596

年卷期： 2019 年 20 卷 16 期

页码：

收录情况： SCI

摘要： The plant hormone jasmonic acid (JA) has been recognized as an important promoter of leaf senescence in plants. However, upstream transcription factors (TFs) that control JA biosynthesis during JA-promoted leaf senescence remain unknown. In this study, we report the possible involvement of a TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) TF BrTCP7 in methyl jasmonate (MeJA)-promoted leaf senescence in Chinese flowering cabbage. Exogenous MeJA treatment reduced maximum quantum yield (Fv/Fm) and total chlorophyll content, accompanied by the increased expression of senescence marker and chlorophyll catabolic genes, and accelerated leaf senescence. To further understand the transcriptional regulation of MeJA-promoted leaf senescence, a class I member of TCP TFs BrTCP7 was examined. BrTCP7 is a nuclear protein and possesses trans-activation ability through subcellular localization and transcriptional activity assays. A higher level of BrTCP7 transcript was detected in senescing leaves, and its expression was up-regulated by MeJA. The electrophoretic mobility shift assay and transient expression assay showed that BrTCP7 binds to the promoter regions of a JA biosynthetic gene BrOPR3 encoding OPDA reductase3 (OPR3) and a chlorophyll catabolic gene BrRCCR encoding red chlorophyll catabolite reductase (RCCR), activating their transcriptions. Taken together, these findings reveal that BrTCP7 is associated with MeJA-promoted leaf senescence at least partly by activating JA biosynthesis and chlorophyll catabolism, thus expanding our knowledge of the transcriptional mechanism of JA-mediated leaf senescence.