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The Effect of Ghrelin on the Maturation of Sheep Oocytes and Early Embryonic Development In Vitro

文献类型： 外文期刊

作者： Wang, Daqing 1 ; Yang, Yanyan 1 ; Song, Yongli 3 ; Fu, Shaoyin 1 ; He, Xiaolong 1 ; Wang, Biao 1 ; Wang, Liwei 1 ; Chen, Xin 1 ; Li, Xihe 3 ; Liu, Yongbin 4 ; Cao, Guifang 2 ;

作者机构： 1.Inner Mongolia Acad Agr & Anim Husb Sci, Inst Anim Husb, Hohhot 010031, Peoples R China

2.Inner Mongolia Agr Univ, Sch Vet Med, Hohhot 010010, Peoples R China

3.Inner Mongolia Univ, Sch Life Sci, Res Ctr Anim Genet Resources Mongolia Plateau, Hohhot 010021, Peoples R China

4.Inner Mongolia Univ, Sch Life Sci, State Key Lab Reprod Regulat & Breeding Grassland, Hohhot 010021, Peoples R China

关键词： ghrelin; sheep oocyte; cell cycle; early embryo; RNA-seq

期刊名称：ANIMALS （ 影响因子：3.231； 五年影响因子：3.312 ）

ISSN： 2076-2615

年卷期： 2022 年 12 卷 9 期

页码：

收录情况： SCI

摘要： Simple Summary Different gradients of ghrelin (0, 100, 200, and 300 ng/mL) were added to the IVM system of sheep oocytes to observe their changes, and 200 ng/mL ghrelin was found to be the optimal concentration. The RNA-seq analysis showed that the Cell cycle signaling pathway was enriched. The results suggest that adding ghrelin shortens the duration of IVM of sheep oocytes and hinders early embryonic development. In vitro maturation (IVM) of sheep oocytes and early embryonic development are of great scientific importance for the study of reproductive development in sheep. Ghrelin is an important hormone that regulates the secretion of the growth hormone (GH). In this study, different gradients of ghrelin (0, 100, 200, and 300 ng/mL) were added to the IVM system of sheep oocytes to observe their cell morphology, and Hosesth 33342 staining was used to determine the time taken for oocytes to reach different developmental stages. We found 200 ng/mL ghrelin to be the optimal concentration. The RNA-seq analysis showed that many signaling pathways were significantly altered by ghrelin. Cell cycle, Wnt, and oxidative phosphorylation were activated; the P53 was inhibited. These pathways together regulate the maturation of oocytes and early embryonic development in vitro. The effects of the addition of ghrelin were verified by the expression of GLUT1 in early embryonic development. The results suggest that adding ghrelin shortens the duration of the IVM of sheep oocytes and hinders early embryonic development. This study provides new insights into the effects of exogenous ghrelin on sheep oocyte maturation and early embryonic development in vitro.

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