A reliable and cost-efficient TLC-HPLC method for determining total florfenicol residues in porcine edible tissues
文献类型： 外文期刊
作者： Zhou, DanNa 1 ; Li, YaChao 2 ; Huang, LingLi 3 ; Qian, MingRong 4 ; Li, Dong 5 ; Sun, GuiZhi 2 ; Yang, Bo 2 ;
作者机构： 1.Hubei Acad Agr Sci, Anim Husb & Vet Inst, Minist Agr, Key Lab Prevent & Control Agents Anim Bacteriosis, Wuhan 430064, Hubei, Peoples R China
2.Wuhan Univ Bioengn, Hubei Engn Res Ctr Viral Vector, 1 Hanshi Rd, Wuhan 430415, Hubei, Peoples R China
3.Huazhong Agr Univ, MOA Key Lab Food Safety Evaluat, Natl Reference Lab Vet Drug Residues, Wuhan 430070, Hubei, Peoples R China
4.Univ Qual & Stand Agroprod, Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China
5.Wuhan Anim Dis Control Ctr, Wuhan 430016, Hubei, Peoples R China
关键词： Total florfenicol residues; Florfenicol amine; High-performance liquid chromatography; Thin layer chromatography; Porcine edible tissues
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2020 年 303 卷
页码：
收录情况： SCI
摘要： It is still a challenge to solve the matrix interferences in veterinary drug residue analysis. In this study, we reported a thin layer chromatography (TLC)-high-performance liquid chromatography (HPLC) method for determining total florfenicol (FF) residues, expressed as florfenicol amine (FFA), in porcine edible tissues. The tissue homogenate were acid-hydrolyzed to liberate the bound residues and convert them into FFA. The hydrolysates were washed with ethyl acetate and subsequently extracted with ethyl acetate under alkaline conditions. The supernatants were concentrated through evaporation, defatted with hexane, purified by TLC and analyzed by HPLC at 225 nm. The optimal developing solvent for TLC purification was ethyl acetate-acetone-ammonium hydroxide mixtures (2:8:0.5, v/v/v). The method was fully validated according to decision 2002/657/EC, and could be used for the routine monitoring of FF residues in pig. TLC showed excellent purification efficiency, and was expected to solve the matrix interferences in veterinary drug residue analysis.
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