您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Impacts of prothioconazole and prothioconazole-desthio on bile acid and glucolipid metabolism: Upregulation of CYP7A1 expression in HepG2 cells

文献类型： 外文期刊

作者： Hu, Lingyu 2 ; Wang, Xiaofang 2 ; Qian, Mingrong 3 ; Zhang, Hu 1 ; Jin, Yuanxiang 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, Zhejiang Prov Key Lab Food Safety, Hangzhou 310021, Peoples R China

2.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310032, Peoples R China

3.Zhejiang Shuren Univ, Interdisciplinary Res Acad, Key Lab Pollut Exposure & Hlth Intervent Zhejiang, Hangzhou 310015, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, 298 Desheng Middle Rd, Hangzhou 310021, Peoples R China

5.Zhejiang Shuren Univ, Interdisciplinary Res Acad, 8 Shuren St, Hangzhou 310015, Peoples R China

6.Zhejiang Univ Technol, Coll Biotechnol & Bioengn, 18 Chaowang Rd, Hangzhou 310014, Peoples R China

关键词： Prothioconazole; Prothioconazole-desthio; HepG2; Bile acid metabolism; Glucolipid metabolism

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 0048-3575

年卷期： 2024 年 198 卷

页码：

收录情况： SCI

摘要： As an efficient triazole fungicide, prothioconazole (PTC) is widely used for the prevention and control of plant fungal pathogens. It was reported that the residues of PTC and prothioconazole-desthio (PTC-d) have been detected in the environment and crops, and the effects of PTC-d may be higher than that of PTC. Currently, PTC and PTC-d have been proven to induce hepatic metabolic disorders. However, their toxic effects on cellular bile acid (BA) and glucolipid metabolism remain unknown. In this study, HepG2 cells were exposed to 1-500 mu M of PTC or PTC-d. High concentrations of PTC and PTC-d were found to induce cytotoxicity; thus, subsequent experimental exposure was conducted at concentrations of 10-50 mu M. The expression levels of CYP7A1 and TG synthesis-related genes and levels of TG and total BA were observed to increase in HepG2 cells. Molecular docking analysis revealed direct interactions between PTC or PTC-d and CYP7A1 protein. To further investigate the underlying mechanisms, PTC and PTC-d were treated to HepG2 cells in which CYP7A1 expression was knocked down using siCYP7A1. It was observed that PTC and PTC-d affected the BA metabolism process and regulated the glycolipid metabolism process by promoting the expression of CYP7A1. In summary, we comprehensively analyzed the effects and mechanisms of PTC and PTC-d on cellular metabolism in HepG2 cells, providing theoretical data for evaluating the safety and potential risks associated with these substances.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台