您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Single-base methylome profiling of the giant kelp Saccharina japonica reveals significant differences in DNA methylation to microalgae and plants

文献类型： 外文期刊

作者： Fan, Xiao 1 ; Han, Wentao 1 ; Teng, Linhong 1 ; Jiang, Peng 6 ; Zhang, Xiaowen 1 ; Xu, Dong 1 ; Li, Chang 7 ; Pellegrini, 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Shandong, Peoples R China

2.Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Shandong, Peoples R China

3.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China

4.Dezhou Univ, Coll Life Sci, Dezhou 253023, Peoples R China

5.Dezhou Univ, Shandong Key Lab Biophys, Dezhou 253023, Peoples R China

6.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China

7.Univ Chinese Acad Sci, Shenzhen, Peoples R China

8.Univ Calif Los Angeles, Inst Genom & Prote, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

9.Flinders Univ S Australia, Biol Sci, GPO Box 2100, Adelaide, SA 5001, Australia

10.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen, Fujian, Peoples R China

11.Univ Nantes, Fac Sci & Tech, CNRS, UMR 6286, 2 Rue Houssiniere, F-44322 Nantes, France

12.Univ East Anglia, Sch Environm Sci, Norwich Res Pk, Norwich NR4 7TJ, Norfolk, England

关键词： brown algae; BS-PCR; DNA methylation; DNMT2; gene expression; life-cycle stages; MeDIP-seq; WGBS-seq

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

年卷期：

页码：

收录情况： SCI

摘要： Brown algae have convergently evolved plant-like body plans and reproductive cycles, which in plants are controlled by differential DNA methylation. This contribution provides the first single-base methylome profiles of haploid gametophytes and diploid sporophytes of a multicellular alga. Although only c. 1.4% of cytosines in Saccharina japonica were methylated mainly at CHH sites and characterized by 5-methylcytosine (5mC), there were significant differences between life-cycle stages. DNA methyltransferase 2 (DNMT2), known to efficiently catalyze tRNA methylation, is assumed to methylate the genome of S. japonica in the structural context of tRNAs as the genome does not encode any other DNA methyltransferases. Circular and long noncoding RNA genes were the most strongly methylated regulatory elements in S. japonica. Differential expression of genes was negatively correlated with DNA methylation with the highest methylation levels measured in both haploid gametophytes. Hypomethylated and highly expressed genes in diploid sporophytes included genes involved in morphogenesis and halogen metabolism. The data herein provide evidence that cytosine methylation, although occurring at a low level, is significantly contributing to the formation of different life-cycle stages, tissue differentiation and metabolism in brown algae.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台