Molecular characterization and functional analysis of Japanese flounder (Paralichthys olivaceus) thbs2 in response to lymphocystis disease virus
文献类型： 外文期刊
作者： Guo, Yanan 1 ; Nan, Xingyu 1 ; Zhang, Xiaoyan 5 ; Wang, Guixing 5 ; Ren, Yuqin 5 ; Wang, Yufen 5 ; Fu, Yuanshuai 1 ; Hou, 1 ;
作者机构： 1.Minist Agr, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China
2.Shanghai Collaborat Innovat Aquat Anim Genet & Br, Shanghai 201306, Peoples R China
3.Shanghai Ocean Univ, Lab Cell & Mol Biol, Shanghai 201306, Peoples R China
4.Minist Agr, Key Lab Aquat Genom, Beijing 100141, Peoples R China
5.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Hebei, Peoples R China
关键词： thbs2; Lymphocystis; Japanese flounder; Antivirus
期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）
ISSN： 1050-4648
年卷期： 2019 年 93 卷
页码：
收录情况： SCI
摘要： In mammals, a matricellular protein, thrombospondin 2 (Thbs2) has been reported to play important roles in modulating cell-matrix interactions, vascular integrity and thrombosis formation. However, the role of gene, thbs2 has not yet been studied in teleost. In the present study, this novel fish gene from Japanese flounder was cloned and its function in resistant to lymphocystis disease virus was elucidated. The Japanese flounder thbs2 encoded a 1176-amino acid protein with 91% identity to medaka. Amino acid sequence indicated that Japanese flounder Thbs2 contained 10 typical conserved domains. The thbs2 was expressed in all stages of embryo development, and in hatched larva stage, its expression was significantly higher than that in other stages (P < 0.05). The relative expression level of thbs2 was significantly higher in the head kidney, liver, blood, gill, and heart of the lymphocystis disease virus resistant fish than in sensitive fish (P < 0.05); and in muscle, this difference was at highly significant (P < 0.01). Additionally, the distribution of Thbs2 in tissue was evaluated by immunohistochemical staining. Subcellular localization analysis showed that Thbs2 was distributed throughout the cytoplasm of the cells. Taken together, our results provide new basic data for thbs2 function, especially its role in anti-lymphocystis disease virus immune response.
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