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Genetic Diversity and Population Structure Analysis of Sand Pear (Pyrus pyrifolia) 'Nakai' Varieties Using SSR and AFLP Markers

文献类型： 外文期刊

作者： Cao, Jie 1 ; Zhou, Zhongcheng 2 ; Tu, Junfan 3 ; Cheng, Shuiyuan 4 ; Yao, Jinglei 1 ; Xu, Feng 1 ; Wang, Guiyuan 1 ; Zhan 1 ;

作者机构： 1.Yangtze Univ, Coll Hort & Gardening, Jingzhou 434025, Hubei, Peoples R China

2.Hubei Ecol Polytech Coll, Dept Forestry Ecol, Wuhan 430070, Hubei, Peoples R China

3.Hubei Acad Agr Sci, Fruit & Tea Res Inst, Wuhan 430064, Hubei, Peoples R China

4.Wuhan Polytech Univ, Natl R&D Ctr Se Rich Agr Prod Proc Technol, Wuhan 430023, Hubei, Peoples R China

5.Chongqing Univ Arts & Sci, Res Inst Special Plants, Chongqing 402160, Peoples R China

关键词： cluster analysis; genetic polymorphism; genetic structure; molecular markers; pear varieties

期刊名称：NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA （ 影响因子：1.444； 五年影响因子：1.363 ）

ISSN： 0255-965X

年卷期： 2019 年 47 卷 3 期

页码：

收录情况： SCI

摘要： In this study, the technologies of simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers were used to analyze the genetic diversity of 30 sand pear (Pyrus pyrifolia) 'Nakai' varieties. Ten pairs of SSR polymorphic primers were selected to amplify P. pyrifolia 'Nakai' varieties. A total of 90 alleles were detected. The polymorphism information content index was between 0.5578 and 0.8423, with an average of 0.7585. The selected 10 pairs of AFLP primer combinations were used to amplify the analyzed pear varieties, and 1,046 polymorphic loci were detected. The average amplification results of each primer combination detected 105 bands with an average polymorphism percentage of 86.46%. The combined data of SSR and AFLP analysis showed that the analyzed P. pyrifolia 'Nakai' varieties were characterized by extremely rich genetic diversity and were highly representative. According to the results of SSR, AFLP, and SSR+AFLP cluster analysis, the analyzed P. pyrifolia 'Nakai' varieties can be categorized into three clusters. The results of genetic structure showed that the hybridization between these P. pyrifolia 'Nakai' varieties resulted in the heterozygosity of genotypes. In addition, we found that 'Nijisseik', 'Ejima', and 'Fuli' are good parent resources among the pear varieties through observing the genetic background of the analyzed pear varieties. This study reveals the genetic diversity levels of P. pyrifolia 'Nakai' varieties at the molecular level, which was important in molecular identification and protection of pear germplasm resources, as well as pear variety breeding and genetic improvement.

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