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Enzymes in the juvenile hormone biosynthetic pathway can be potential targets for pest control

文献类型： 外文期刊

作者： Yin, Yue 1 ; Qiu, Yan-Wei 2 ; Huang, Juan 3 ; Tobe, Stephen S. 4 ; Chen, Shan-Shan 1 ; Kai, Zhen-Peng 2 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Test Technol, Shanghai 201403, Peoples R China

2.Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai 201418, Peoples R China

3.Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects, Beijing, Peoples R China

4.Univ Toronto, Dept Cell & Syst Biol, Toronto, ON, Canada

5.China Agr Univ, Coll Sci, Dept Appl Chem, Beijing, Peoples R China

关键词： JH biosynthesis; RNAi; target; Manduca sexta

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期：

页码：

收录情况： SCI

摘要： BACKGROUND Discovery of novel insecticides and targets has received global attention in recent years. Ten genes coding for enzymes involved in the juvenile hormone biosynthetic pathway of Manduca sexta were studied as potential insecticide targets. RESULTS We determined the expression of genes encoding some critical enzymes in the JH biosynthetic pathway. Farnesol dehydrogenase (FOLD), Juvenile hormone acid O-methyltransferase (JHAMT) and Juvenile hormone epoxidase (CYP15C1) were selected as the candidate targets based on gene expression results. RNAi silencing and enzyme inhibitor tests were performed to validate whether these candidate genes could be the potential insecticide targets. The down-regulation of FOLD, JHAMT and CYP15C1 resulted in a 68%, 82% and 79% reduction in the rates of JH biosynthesis in vitro, respectively. In addition, RNA interference and inhibitor studies of these enzymes following oral administration demonstrated the potential application in pest management, with respect to high mortality and effects on growth. CONCLUSION Based on our study, FOLD, JHAMT and CYP15C1 could be potential targets for pest control as a consequence of their important roles in insect development. (c) 2019 Society of Chemical Industry

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