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RNA polymerase II-independent recruitment of SPT6L at transcription start sites in Arabidopsis

文献类型： 外文期刊

作者： Chen, Chen 1 ; Shu, Jie 1 ; Li, Chenlong 3 ; Thapa, Raj K. 1 ; Vi Nguyen 1 ; Yu, Kangfu 4 ; Yuan, Ze-Chun 1 ; Kohalmi, Su 1 ;

作者机构： 1.Agr & Agri Food Canada, London Res & Dev Ctr, London, ON N5V 4T3, Canada

2.Western Univ, Dept Biol, London, ON N6A 5B7, Canada

3.Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Plant Resource, Guangzhou 510275, Guangdong, Peoples R China

4.Agr & Agri Food Canada, Harrow Res & Dev Ctr, Harrow, ON N0R 1G0, Canada

5.Guangdong Acad Agr Sci, Guangzhou 510640, Guangdong, Peoples R China

期刊名称：NUCLEIC ACIDS RESEARCH （ 影响因子：16.971； 五年影响因子：15.542 ）

ISSN： 0305-1048

年卷期： 2019 年 47 卷 13 期

页码：

收录情况： SCI

摘要： SPT6 is a conserved elongation factor that is associated with phosphorylated RNA polymerase II (RNAPII) during transcription. Recent transcriptome analysis in yeast mutants revealed its potential role in the control of transcription initiation at genic promoters. However, the mechanism by which this is achieved and how this is linked to elongation remains to be elucidated. Here, we present the genome-wide occupancy of Arabidopsis SPT6-like (SPT6L) and demonstrate its conserved role in facilitating RNAPII occupancy across transcribed genes. We also further demonstrate that SPT6L enrichment is unexpectedly shifted, from gene body to transcription start site (TSS), when its association with RNAPII is disrupted. Protein domains, required for proper function and enrichment of SPT6L on chromatin, are subsequently identified. Finally, our results suggest that recruitment of SPT6L at TSS is indispensable for its spreading along the gene body during transcription. These findings provide new insights into the mechanisms underlying SPT6L recruitment in transcription and shed light on the coordination between transcription initiation and elongation.

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