文献类型： 外文期刊

作者： Yang, G. D. ¹ ; Zhou, Y. F. ¹ ; Huang, R. D. ¹ ; Lin, F. ² ; Hu, Z. Y. ³ ; Hao, Z. Y. ³ ; Liang, C. B. ⁴ ; Wang, Q. ⁵ ; Meng ¹ ;

作者机构： 1.Shenyang Agr Univ, Coll Agron, 120 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China

2.Shenyang Agr Univ, Coll Biosci & Biotechnol, 120 Dongling Rd, Shenyang 110866, Liaoning, Peoples R China

3.Heilongjiang Acad Agr Sci, Keshan Branch, Harbin 161005, Heilongjiang, Peoples R China

4.Heilongjiang Acad Agr Sci, Ind Crops Inst, Harbin 150086, Heilongjiang, Peoples R China

5.Heilongjiang Acad Agr Sci, Crop Resources Inst, Harbin 150086, Heilongjiang, Peoples R China

6.Guangzhou Univ, Sch Life Sci, Guangzhou 510006, Guangdong, Peoples R China

关键词： sorghum; N tolerance; chlorophyll; transcriptome; differentially expressed genes; candidate genes

期刊名称：APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH （ 影响因子：0.711； 五年影响因子：0.796 ）

ISSN： 1589-1623

年卷期： 2019 年 17 卷 5 期

页码：

收录情况： SCI

摘要： Sorghum [Sorghum bicolor (L.) Moench] is an important cereal crop with high nitrogen utilization efficiency. In order to analyze the mechanisms of different sorghum genotypes to low-N tolerance and provide the theoretical basis for the breeding of low nitrogen tolerant sorghum varieties, two materials (KZ15 and SZ7) with different tolerance to low-N were treated with nitrogen by hydroponics, the phenotypes were observed, physiological indexes were measured and transcripts were analyzed in this study. Compared to SZ7, KZ15 showed significantly higher capacity of low-N tolerance. The results showed that the change of KZ15 chlorophyll content was lower than that of SZ7 under low-N stress. The activity of nitrate reductase (NR) and glutamine synthetase (GS) had a smaller reduction in KZ15 than SZ7 under low-N stress. High-throughput sequencing revealed several common differentially expressed genes (DEGs) between KZ15 and SZ7, including nitrogen transport and nitrate assimilation genes. In the tolerant genotype KZ15, there were more transcripts related to high affinity nitrate transporters (NRT2.4, NRT3.1 and NRT4.5), suggesting an improved uptake efficiency of inorganic and organic forms of nitrogen. In addition, an abundance of certain transcription factors in the tolerant genotype KZ15 suggests that they may play roles in regulating the response to N-stress. This study will enable us to discover candidate genes potentially useful in improving the resistance to low nitrogen stress by genetic engineering technology and provide theoretical basis for the breeding of low nitrogen tolerant sorghum varieties.