文献类型： 外文期刊
作者： Hu, Chun-hua 1 ; Yang, Qiao-song 1 ; Shao, Xiu-hong 1 ; Dong, Tao 1 ; Bi, Fang-cheng 1 ; Li, Chun-yu 1 ; Deng, Gui-min 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Fruit Tree Res, 80 Dafeng 2nd St, Guangzhou 510640, Guangdong, Peoples R China
2.Minist Agr, Key Lab South Subtrop Fruit Biol & Genet Resource, Guangzhou, Guangdong, Peoples R China
3.Key Lab Trop & Subtrop Fruit Tree Res, Guangzhou 510640, Guangdong, Peoples R China
4.Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China
5.Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT 06269 USA
关键词： Gene-deletor; Transgenic; Arabidopsis; Specific promoter; Leafy
期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）
ISSN： 0167-6857
年卷期：
页码：
收录情况： SCI
摘要： Key message These results suggest that LFY promoter could not completely drive the 'Gene-deletor' vector to achieve the effect of complete elimination of exogenous gene in bananas. Nevertheless, a certain effect of exogenous gene elimination laid a theoretical foundation for the next step of screening banana fruit-specific promoters, removing all exogenous genes from banana fruits, and solving the food safety problem of genetically modified bananas. Banana (Musa spp.) is an important tropical crop. Banana industry is under biotic and abiotic stresses such as Fusarium wilt, typhoon, cold stress. Genetic engineering offers a powerful strategy to create germplasm of banana with enhanced resistance. The safety of genetically modified organisms has become a bottleneck restricting the popularization and application of genetically modified technology. In this study, a candidate promoter, LEAFY (LFY) for expression and flower initiation in Arabidopsis, was cloned and constructed to 'Gene-deletor' vector. Histochemical beta-glucuronidase (GUS) staining results showed that the 'Gene-deletor' vector driven by LFY promoter could lead to 88.5% excision efficiency from Arabidopsis seeds based on more than 200 T-3 progeny examined per event. GUS staining was found to be partially negative in transgenic bananas, however, polymerase chain reaction could still detect the presence of large fragments of the vector. These results suggest that although LFY promoter could not completely drive the 'Gene-deletor' vector to achieve the effect of complete elimination of exogenous gene in bananas, its efficiency of eliminating exogenous gene laid a theoretical foundation for cloning banana fruit-specific promoters, that is, 'non-transgenic' GM bananas.
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