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Remediation and Mechanisms of Cadmium Biosorption by a Cadmium-Binding Protein from Lentinula edodes

文献类型： 外文期刊

作者： Dong, Xiao-bo 1 ; Huang, Wen 1 ; Bian, Yin-bing 2 ; Feng, Xi 3 ; Ibrahim, Salam A. 4 ; Shi, De-fang 1 ; Qiao, Xin 6 ; Liu 1 ;

作者机构： 1.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China

2.Huazhong Agr Univ, Inst Appl Mycol, Wuhan 430070, Hubei, Peoples R China

3.Calif State Univ, Dept Nutr Food Sci & Packaging, San Jose, CA 95192 USA

4.North Carolina A&T State Univ, Dept Family & Consumer Sci, 171 Carver Hall, Greensboro, NC 27411 USA

5.Hubei Acad Agr Sci, Res Inst Agr Prod Proc & Nucl Agr Technol, Wuhan 430064, Hubei, Peoples R China

6.Wuhan Business Univ, Coll Culinary & Food Engn, Wuhan 430056, Hubei, Peoples R China

关键词： Lentinula edodes; cadmium; cadmium-binding protein; bioremediation; interaction

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2019 年 67 卷 41 期

页码：

收录情况： SCI

摘要： Cadmium bioremediation with metal-binding proteins is primarily conducted using metallothioneins (MTs). However, in the present study, we investigated a non-MT cadmium-binding protein from Lentinula edodes (LECBP) as a remediation tool for cadmium biosorption in Escherichia coli. The results indicated that the expression of LECBP significantly enhanced the cadmium biosorption capacity of transgenic E. coli. The secondary structure and conformation of LECBP were changed after binding with cadmium as evidenced by circular dichroism and fluorescence spectroscopy. The results of Fourier transform infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were involved in the interaction between LECBP and cadmium. The results further demonstrated that glutamic acid and histidine residues are the potential binding sites. Our results have thus provided new insights into cadmium bioremediation in an aquatic environment.

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