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Acidic conditions induce the accumulation of orange Monascus pigments during liquid-state fermentation of Monascus ruber M7

文献类型： 外文期刊

作者： Li, Li 1 ; Chen, Suo 1 ; Gao, Mengxiang 1 ; Ding, Baomiao 1 ; Zhang, Jialan 2 ; Zhou, Youxiang 3 ; Liu, Yingbao 1 ; Yang, 1 ;

作者机构： 1.Yangtze Univ, Coll Life Sci, Jingzhou 434025, Hubei, Peoples R China

2.Yangtze Univ, Coll Anim Sci, Jingzhou 434025, Hubei, Peoples R China

3.Hubei Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Wuhan 430064, Hubei, Peoples R China

4.Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Wuhan 434070, Hubei, Peoples R China

5.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 434070, Hubei, Peoples R China

关键词： Monascus ruber; Orange Monascus pigments; pH regulation; Biosynthesis genes

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2019 年 103 卷 20 期

页码：

收录情况： SCI

摘要： The influence of pH on the biosynthesis of orange Monascus pigments (OMPs) in Monascus ruber M7 was investigated. Under acidic fermentation conditions, pigment mixtures predominantly rich in OMPs were obtained. HPLC analysis revealed the presence of four orange components (O1-O4) and four yellow components (Y1-Y4) in the mixtures, and the dominant ones were O1 and O3, which accounted for 56.0% to 75.9% of the total pigments in the pH range 3-6. Subsequently, O1 and O3 were identified by LC-DAD-ESI/MS as Rubropunctatin and Monascorubrin, respectively. The yield of OMPs was observed to be inversely dependent on pH. At pH 3, large amounts of OMPs with high purity (79.1%) were accumulated. A real-time quantitative PCR analysis revealed that the expression of genes related to the biosynthesis of OMPs in M. ruber M7 was upregulated at acidic pH as compared to neutral pH, and the variation in the level of expression of these genes with pH was consistent with the production of OMPs. These results indicated that the large accumulation of OMPs under acidic condition involved the acidic pH-induced transcription of genes related to the biosynthesis of OMPs. These results would contribute towards the development of an efficient technology for large-scale production of OMPs.

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