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Transcriptome analysis in different chieh-qua cultivars provides new insights into drought-stress response

文献类型： 外文期刊

作者： Wang, Min 1 ; He, Xiaoming 1 ; Jiang, Biao 1 ; Liu, Wenrui 1 ; Lin, Yu'e 1 ; Xie, Dasen 1 ; Liang, Zhaojun 1 ; Chen, Lihu 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou, Guangdong, Peoples R China

2.Guangdong Key Lab New Technol Res Vegetables, Guangzhou, Guangdong, Peoples R China

3.Zhaoqing Univ, Coll Life Sci, Zhaoqing, Peoples R China

关键词： RNA-sequencing; Chieh-qua; Drought; Plant hormone

期刊名称：PLANT BIOTECHNOLOGY REPORTS （ 影响因子：2.01； 五年影响因子：1.907 ）

ISSN： 1863-5466

年卷期：

页码：

收录情况： SCI

摘要： Drought, one of the crucial environmental constraints, seriously threats the quality and yield in chieh-qua. Therefore, cultivating drought-tolerant variety is greatly necessary for its normal growth under water deficiency. However, at present, molecular knowledge on drought resistance is mostly unclear in chieh-qua. In the study, characteristics of two diverse genetic chieh-qua variety, A39 (drought-resistance) and H5 (drought-sensitivity), were analyzed. Under drought stress, H5 exerted high water loss rate, increased malonaldehyde (MDA) content, and decreased enzyme activity of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) compared with A39. In addition, based on the transcriptome results, we obtained a total of 1821 (511 up-regulated and 1310 down-regulated) and 2114 (1282 up-regulated and 832 down- regulated) differentially expressed genes (DEGs) in the A39 versus H5 under normal and water-deficiency stress, respectively. Several DEGs involved in the cuticle synthesis (cytochrome P450 genes: CYP94A2, CYP86B1, CYP86A7), carbohydrate metabolism, and plant hormone signal transduction (small auxin-up RNA genes: SAUR32, SAUR72; JA-induced genes: TIFY 10A, TIFY 10C; ABA related genes: PYL2, PYL4) were explored and related to drought resistance. These expression patterns observed in the RNA-seq data were further confirmed with quantitative real-time PCR (qRT-PCR). In all, these results not only provided a new insight into analyzing genes of drought response, but also laid a foundation for isolating crucial genes involved in drought stress in chieh-qua.

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[1]Transcriptome analysis in different chieh-qua cultivars provides new insights into drought-stress response. Wang, Min,He, Xiaoming,Jiang, Biao,Liu, Wenrui,Lin, Yu'e,Xie, Dasen,Liang, Zhaojun,Peng, Qingwu,Wang, Min,He, Xiaoming,Jiang, Biao,Xie, Dasen,Chen, Lihui. 2019

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