Molecular typing of Streptococcus agalactiae isolates of serotype Ia from tilapia in southern China
文献类型： 外文期刊
作者： Su, Youlu 1 ; Liu, Chan 1 ; Deng, Yiqin 1 ; Cheng, Changhong 1 ; Ma, Hongling 1 ; Guo, Zhixun 1 ; Feng, Juan 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China
2.Tianjin Agr Univ, Coll Fisheries, Tianjin 300384, Peoples R China
关键词： Streptococcus agalactiae; serotype Ia; tilapia; virulence gene profiles; genetic variation; multi-virulence locus sequencing typing
期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）
ISSN： 0378-1097
年卷期： 2019 年 366 卷 13 期
页码：
收录情况： SCI
摘要： Streptococcus agalactiae is an important pathogen of tilapia causing enormous economic losses worldwide. In this study, multilocus sequence typing indicated that 75 S. agalactiae isolates from tilapia in southern China belonged to sequence type-7, as well as belonging to serotype Ia, as confirmed by multiplex PCR assay. The putative-virulence gene profiles and genetic variation of these strains were determined by three sets of multiplex PCR and multi-virulence locus sequencing typing (MVLST), respectively. Analysis of putative-virulence gene profiles showed that each strain harbored 18 putative-virulence genes but lacked lmb and scpB. Three putative-virulence genes (srr-1, bibA and fbsA) were further selected for MVLST analysis. Our data showed that the strains had 14 MVLST types (1-14) and clustered in three groups (Groups I-III). The period of time during 2013 and 2014 was an important turning point for the differentiation of the putative-virulence genes of S. agalactiae, as type 1 within Group II became the predominant MVLST type. There were significant differences in MVLST types of S. agalactiae isolated from different tilapia farming regions. MVLST assay may improve the discriminatory power and is suitable for understanding the epidemiology of S. agalactiae serotype Ia and screening multivalent vaccine candidate strains.
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