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Liver proteome analysis of grass carp (Ctenopharyngodon idellus) following treatment with enrofloxacin

文献类型： 外文期刊

作者： Ma, Rongrong 1 ; Fang, Wenhong 2 ; Yang, Zhongying 3 ; Hu, Kun 4 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China

2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Minist Agr, Key Lab East China Sea Fishery Resources Exploita, Shanghai 200090, Peoples R China

3.Nanchang Acad Agr Sci, Nanchang 330038, Jiangxi, Peoples R China

4.Shanghai Ocean Univ, Coll Aquat & Life, Shanghai 201306, Peoples R China

5.Shanghai Ocean Univ, Coll Fisheries & Life Sci, 999 Hucheng Huan Rd, Shanghai 201306, Peoples R China

关键词： Proteome; Grass carp; Liver; Enrofloxacin; Hepatotoxicity

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2019 年 45 卷 6 期

页码：

收录情况： SCI

摘要： Enrofloxacin is widely used for the prevention and control of bacterial diseases in aquaculture. The liver is crucial for enrofloxacin metabolism, but enrofloxacin can induce liver damage. Herein, we explored proteomic changes in the liver of grass carp (Ctenopharyngodon idellus) following treatment with enrofloxacin using isobaric tag for relative and absolute quantitation (iTRAQ) technology. All experiments included two biological replicates and blank controls. Among the 3082 proteins identified, 103 were differentially abundant, comprising 49 up- and 54 downregulated proteins. Gene Ontology (GO) annotation identified macromolecular complex (63.60%), intracellular non-membrane-bound organelle (51.50%), and non-membrane-bound organelle (51.50%) as the most enriched cellular component terms. Structural molecule activity (26.80%), structural constituent of ribosome (17.90%), and calcium ion binding (16.10%) were the top three molecular function terms. Organic substance biosynthetic process (37.80%), biosynthetic process (37.80%), and protein metabolic process (37.80%) were the top three biological process terms. The Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analysis found 17 enriched KEGG pathways, with protein digestion and absorption, extracellular matrix (ECM)-receptor interactions, and ribosome and focal adhesion the most significant (p < 0.001). Analysis of the most enriched pathways revealed that chymotrypsin-like precursor, pancreatic elastase precursor, Na+/K+ transporting ATPase, collagen, and dermatopontin were upregulated, while ribosomal proteins, alpha-actinin, and myosin light chain were downregulated. These findings suggest that enrofloxacin affects liver function and has a risk of inducing an inflammatory response in extrahepatic organs.

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