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Development and Evaluation of a Novel Armored RNA Technology Using Bacteriophage Q beta

文献类型： 外文期刊

作者： Yao, Lin 1 ; Li, Fengling 1 ; Qu, Meng 1 ; Guo, Yingying 1 ; Jiang, Yanhua 1 ; Wang, Lianzhu 1 ; Zhai, Yuxiu 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qua, Qingdao, Shandong, Peoples R China

关键词： Armored RNA; Q beta phage; Stability; Comparison; Norovirus

期刊名称：FOOD AND ENVIRONMENTAL VIROLOGY （ 影响因子：2.778； 五年影响因子：2.799 ）

ISSN： 1867-0334

年卷期： 2019 年 11 卷 4 期

页码：

收录情况： SCI

摘要： Foodborne viruses are a global threat to food safety. Real-time reverse transcription polymerase chain reaction (RT-PCR) is the most commonly used method to detect viral RNA in food. Armored RNA (AR) prepared using the MS2 phage system is a successful positive control for detecting foodborne viruses and is an important quality control process when using real-time RT-PCR. In this study, we report a novel technology for preparing AR using bacteriophage Q beta and compare its stability with AR prepared using the MS2 phage system for packaging norovirus detection target RNA. AR could be successfully and efficiently produced using the developed bacteriophage Q beta system. Two types of AR-AR-QNoV prepared using the Q beta system and AR-MNoV prepared using the MS2 system-were stored at different temperatures for different durations. After incubating at - 20 degrees C for 360 days, the copy numbers of AR-QNoV and AR-MNoV decreased by 8.9% and 35.9%, respectively. After incubating at 4 degrees C for 60 days, the copy numbers of AR-QNoV and AR-MNoV decreased by 12.0% and 38.9%, respectively. After incubating at 45 degrees C, the copy numbers of AR-QNoV decreased by 71.8% after 5 days, whereas those of AR-MNoV decreased by 92.9% after only 4 days. After 5 days, AR-MNoV could not be detected using real-time RT-PCR. There was a significant difference in copy numbers decrease rate between AR-QNoV and AR-MNoV at three different temperatures (P < 0.05 ). Therefore, AR prepared using the new bacteriophage Q beta system is more stable than the traditional AR, making the developed strategy a good candidate for AR preparation and quality control.

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