Development of a Real-Time PCR Method for Identifying Four Major Freshwater Fish in Marine Fish Surimi
文献类型： 外文期刊
作者： Li, Yutong 1 ; Qu, Meng 2 ; Liu, Enchong 2 ; Wan, Shuo 4 ; Jiang, Yanhua 2 ; Guo, Yingying 2 ; Zhu, Wenjia 2 ; Li, Na 2 ; Wang, Lianzhu 2 ; Yao, Lin 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Minist Agr & Rural Affairs, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China
3.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
4.Anjoy Foods Grp Co Ltd, Xiamen 361028, Peoples R China
关键词： Marine fish surimi; Freshwater fish; Identification; ND5 gene; QPCR
期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.0； 五年影响因子：3.1 ）
ISSN： 1936-9751
年卷期： 2025 年 18 卷 10 期
页码：
收录情况： SCI
摘要： BackgroundThe adulteration of marine fish surimi with freshwater fish without consumer disclosure is a growing concern in China. Thus, a rapid detection method is needed for four major freshwater species: black carp (Mylopharyngodon piceus), grass carp (Ctenopharyngodon idella), silver carp (Hypophthalmichthys molitrix), and bighead carp (Hypophthalmichthys nobilis).Methods and ResultsMitochondrial NADH dehydrogenase subunit 5 (ND5) sequences were aligned and analyzed to design species-specific primers and probes for a real-time quantitative polymerase chain reaction (qPCR) assay. This method enabled the simultaneous identification of M. piceus, C. idella, H. molitrix, and H. nobilis in marine fish surimi. The designed primers and probes demonstrated high specificity, with no cross-reactivity to 24 other fish species in the qPCR reaction. The detection limits of the method were 0.0005 ng mu L-1 for M. piceus and H. nobilis and 0.005 ng mu L-1 for C. idella and H. molitrix. The method detected M. piceus at 0.1%, C. idella at 0.01%, and both H. molitrix and H. nobilis at 0.001% in fish mixtures. Among 50 commercial samples, 31 tested positive for one or more of these species.ConclusionThe developed qPCR method specifically detects M. piceus, C. idella, H. molitrix, and H. nobilis in marine fish surimi and has potential for use in routine quality control by food regulators.
- 相关文献
作者其他论文 更多>>
-
Rational Design of Alginate Lyase AlgL3199 for Thermal Stability and Specific Activity Enhancement
作者：Chen, Zhifang;Hao, Jianhua;Guo, Yingying;Zhu, Tiantian;Sun, Jingjing;Wang, Wei;Jiang, Chengcheng;Wang, Haiying;Chen, Zhifang;Hao, Jianhua
关键词：alginate lyase; thermal stability; site-directedmutagenesis; molecular docking
-
Establishment and Characteristics of the Spermatogonial Stem Cell Line from the Yellow River Carp (Cyprinus carpio haematopterus)
作者：Zhou, Huijie;Liu, Tianqi;Zhang, Tan;Sun, Zhipeng;Xu, Huan;Zhang, Tingting;Yin, Yashan;Li, Na;Yan, Ting;Kuang, Youyi;Zhou, Huijie;Liu, Tianqi;Zhang, Tan;Sun, Zhipeng;Xu, Huan;Zhang, Tingting;Yin, Yashan;Li, Na;Yan, Ting;Kuang, Youyi;Zhou, Huijie;Liu, Tianqi;Zhang, Tan;Sun, Zhipeng;Xu, Huan;Zhang, Tingting;Yin, Yashan;Li, Na;Yan, Ting;Kuang, Youyi;Zhou, Huijie;Yin, Yashan;Li, Na
关键词：
Cyprinus carpio haematopterus ; gonad stem cell culture; spermatogenesis; in vitro differentiation; transplantation -
Inclusion of macroalgae in the diet - a comparative survey from Norway, Chile and China
作者：Goecke, Franz;Ergon, Ashild;Aakre, Inger;Angarita, Lisse;Escobar, Maria Cristina;Cisternas, Silvana;Li, Na;Wang, Lianzhu;Li, Xiaodong;Pang, Shaojun
关键词：seaweed; nutrition; seafood; iodine; food risks; culinary traditions
-
Explainable Deep Learning to Predict Kelp Geographical Origin from Volatile Organic Compound Analysis
作者：Kang, Xuming;Tan, Zhijun;Zhao, Yanfang;Yao, Lin;Sheng, Xiaofeng;Guo, Yingying;Tan, Zhijun
关键词：kelp; volatile organic compounds; explainable deep learning; SHAP; geographical origin
-
Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme
作者：Zhao, Yanfang;Guo, Yingying;Kang, Xuming;Sheng, Xiaofeng;Tan, Zhijun;Wu, Jifa
关键词：Sargassum fusiforme; Toxic elements; Bioaccumulation; Detoxification; Chemical forms; Transcriptomic analysis
-
Accumulation Patterns and Health Risk Assessment of Trace Elements in Intermuscular Bone-Free Crucian Carp
作者：Tang, Shizhan;Li, Na;Sun, Zhipeng;Yan, Ting;Zhang, Tingting;Xu, Huan;Chen, Zhongxiang;Qin, Dongli;Kuang, Youyi;Tang, Shizhan;Chen, Zhongxiang;Qin, Dongli
关键词：trace elements; gene-edited fish; health risk assessment; pollution index; crucian carp
-
Integrated evidence of transcriptional, metabolic, and intestinal microbiota changes in Ruditapes philippinarum due to perfluorooctanoic acid-induced immunotoxicity
作者：Li, Fengling;Gong, Xiuqiong;Zhou, Yang;Geng, Qianqian;Jiang, Yanhua;Yao, Lin;Qu, Meng;Tan, Zhijun;Gong, Xiuqiong;Zhou, Yang;Tan, Zhijun
关键词：Perfluorooctanoic acid; Immunotoxicity; Intestine microbiota; Metabolome; Transcriptome; Bivalve
