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Digital gene expression analysis in the liver of ScpB-vaccinated and Streptococcus agalactiae-challenged Nile tilapia

文献类型： 外文期刊

作者： Ke, Xiao-Li 1 ; Zhang, De-Feng 1 ; Li, Qing-Yong 3 ; Liu, Zhi-Gang 1 ; Gao, Feng-Ying 1 ; Lu, Mai-Xin 1 ; Yang, Hong 2 ;

作者机构： 1.Chinese Acad Fisheries Sci, Pearl River Fisheries Res Inst, Key Lab Aquat Anim Immune Technol Guangdong Prov, Minist Agr,Key Lab Trop & Subtrop Fishery Resourc, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 516002, Jiangsu, Peoples R China

3.Fisheries Res & Extens Ctr Huizhou, Huizhou 516002, Peoples R China

关键词： Oreochromis niloticus; Digital gene expression; Streptococcus agalactiae; ScpB vaccine; Liver

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2019 年 94 卷

页码：

收录情况： SCI

摘要： In recent years, streptococcal diseases have severely threatened the development of tilapia aquaculture, but effective prevention and control methods have not yet been established. To understand the immune responses of vaccinated Nile tilapia (Oreochromis niloticus), digital gene expression (DGE) technology was applied in this study to detect the gene expression profile of the Nile tilapia (O. niloticus) liver in response to ScpB (Streptococcal C5a peptidase from group B Streptococcus, ScpB) vaccination and a Streptococcus agalactiae-challenge. The control and the ScpB-vaccinated Nile tilapia yielded a total of 25,788,734 and 27,088,598 clean reads, respectively. A total of 1234 significant differentially expressed unigenes were detected (P < 0.05), of which 236 were significantly up-regulated, and 269 were significantly down-regulated (P < 0.05, vertical bar fold vertical bar > 2, FDR < 0.05). Of the differentially expressed gene, the identified genes which were enriched using databases of GO and KEGG could be categorized into a total of 67 functional groups and were mapped to 153 signaling pathways including 15 immune-related pathways. The differentially expressed genes (TLR1, TLR2, TLR3, TLR5, TLR9, MyD88, C3, IL-1 beta IL-10) were detected in the expression profiles, and this was subsequently verified via quantitative real-time PCR (qPCR). The results of this study can serve as a basis for future research not only on the molecular mechanism of S. agalactiae invasion, but also on the anti-S. agalactiae mechanism in targeted tissues of Nile tilapia.

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