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Changes in the Carbon Metabolism of Escherichia coli During the Evolution of Doxycycline Resistance

文献类型： 外文期刊

作者： Yang, Yiwen 1 ; Mi, Jiandui 1 ; Liang, Jiadi 1 ; Liao, Xindi 1 ; Ma, Baohua 5 ; Zou, Yongde 5 ; Wang, Yan 1 ; Liang, Juan 1 ;

作者机构： 1.South China Agr Univ, Coll Anim Sci, Natl Engn Res Ctr Breeding Swine Ind, Guangzhou, Guangdong, Peoples R China

2.South China Agr Univ, Minist Agr, Key Lab Trop Agr Environm, Guangzhou, Guangdong, Peoples R China

3.Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China

4.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China

5.Nanhai Off, Foshan Customs House, Foshan, Peoples R China

6.Univ Putra Malaysia, Inst Trop Agr, Lab Anim Prod, Serdang, Malaysia

关键词： carbon metabolism; evolution; antibiotic resistance; DOX; Escherichia coli

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Despite our continuous improvement in understanding the evolution of antibiotic resistance, the changes in the carbon metabolism during the evolution of antibiotic resistance remains unclear. To investigate the evolution of antibiotic resistance and the changes in carbon metabolism under antibiotic pressure, Escherichia coli K-12 was evolved for 38 passages under a concentration gradient of doxycycline (DOX). The 0th-passage sensitive strain W0, the 20th-passage moderately resistant strain M20, and the 38th-passage highly resistant strain E38 were selected for the determination of biofilm formation, colony area, and carbon metabolism levels, as well as genome and transcriptome sequencing. The MIC of DOX with E. coli significantly increased from 4 to 96 mu g/ml, and the IC50 increased from 2.18 +/- 0.08 to 64.79 +/- 0.75 mu g/ml after 38 passages of domestication. Compared with the sensitive strain W0, the biofilm formation amount of the resistant strains M20 and E38 was significantly increased (p < 0.05). Single-nucleotide polymorphisms (SNPs) were distributed in antibiotic resistance-related genes such as ribosome targets, cell membranes, and multiple efflux pumps. In addition, there were no mutated genes related to carbon metabolism. However, the genes involved in the biosynthesis of secondary metabolites and carbon metabolism pathway were downregulated, showing a significant decrease in the metabolic intensity of 23 carbon sources (p < 0.05). The results presented here show that there may be a correlation between the evolution of E. coli DOX resistance and the decrease of carbon metabolism, and the mechanism was worthy of further research, providing a theoretical basis for the prevention and control of microbial resistance.

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