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Anthocyanin Fruit encodes an R2R3-MYB transcription factor, SlAN2-like, activating the transcription of SlMYBATV to fine-tune anthocyanin content in tomato fruit

文献类型： 外文期刊

作者： Yan, Shuangshuang 1 ; Chen, Na 1 ; Huang, Zejun 3 ; Li, Dongjing 1 ; Zhi, Junjie 1 ; Yu, Bingwei 1 ; Liu, Xiaoxi 4 ; Cao, 1 ;

作者机构： 1.South China Agr Univ, Coll Hort, Minist Agr, Key Lab Hort Crop Biol & Germplasm Innovat South, Guangzhou 510642, Guangdong, Peoples R China

2.South China Agr Univ, Guangdong Vegetable Engn & Technol Res, Guangzhou 510642, Guangdong, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Minist Agr, Key Lab Biol & Genet Improvement Hort Crops, Beijing 100086, Peoples R China

4.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetable, Guangzhou 510640, Guangdong, Peoples R China

关键词： Anthocyanin fruit (Aft); anthocyanins; atroviolacea (atv); R3-MYB repressors; tomato

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

年卷期： 2020 年 225 卷 5 期

页码：

收录情况： SCI

摘要： Anthocyanin fruit (Aft) and atroviolacea (atv) were characterized in wild tomato and can enhance anthocyanin content in tomato fruit. However, the gene underlying the Aft locus and the mechanism by which Aft and atv act remain largely unknown. In this study, the Aft locus was fine-mapped to an approximately 145-kb interval on chromosome 10, excluding SlAN2 (Solyc10g086250), SlANT1 (Solyc10g086260) and SlANT1-like (Solyc10g086270), which have previously been suggested as candidates. Thus, the R2R3-MYB transcription factor SlAN2-like (Solyc10g086290) was considered the best candidate gene for Aft. The CRISPR/Cas9-mediated SlAN2-like mutants show a much lower accumulation of anthocyanins associated with the downregulation of multiple anthocyanin-related genes compared to the wild-type tomato, indicating that SlAN2-like is responsible for the Aft phenotype. The repressive function of SlMYBATV also was confirmed through the CRISPR/Cas9 approach. A yeast-two-hybrid assay revealed that SlMYBATV interacts with the bHLH protein SlJAF13. Furthermore, yeast-one-hybrid and dual-luciferase transient expression assays showed that Aft directly binds to the SlMYBATV promoter and activates its expression. The results herein provide candidate genes to enhance anthocyanin content in tomato fruit. This research also provides insight into a mechanism involving the Aft-SlMYBATV pathway that fine-tunes anthocyanin accumulation in tomato fruit.

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