Genetic variation analysis of field isolates of clubroot and their responses to Brassica napus lines containing resistant genes CRb and PbBa8.1 and their combination in homozygous and heterozygous state
文献类型： 外文期刊
作者： Shah, Nadil 1 ; Sun, Jincai 3 ; Yu, Shaowei 4 ; Yang, Zhaochun 4 ; Wang, Zuo 4 ; Huang, Fan 1 ; Dun, Bicheng 1 ; Gong, Ji 1 ;
作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
3.Hubei Acad Agr Sci, Inst Econ Crops, Wuhan 430072, Peoples R China
4.Tengchong Agr Technol Extens Inst, Baoshan 679100, Peoples R China
5.Yunnan Agr Acad Sci, Ind Crops Inst, Kunming 650205, Peoples R China
6.Huanggang Normal Univ, Collaborat Innovat Ctr Characterist Resources Exp, Huanggang 438000, Hubei, Peoples R China
7.Huanggang Normal Univ, Coll Biol & Agr Resource, Huanggang 438000, Hubei, Peoples R China
关键词： Brassica napus; CR genes; Genetic variation; Plasmodiophora brassicae; SSR markers
期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）
ISSN： 1380-3743
年卷期： 2019 年 39 卷 11 期
页码：
收录情况： SCI
摘要： Brassica napus is one of the most important oilseed crops in the world and Plasmodiophora brassicae is a serious threat causing yield reduction. CRb and PbBa8.1 resistance genes are known to be highly effective against P. brassicae race 4. Here, we combined two clubroot resistance (CR) genes through marker-assisted selection (MAS) and developed CR homozygous lines. The CR parental and pyramided lines were crossed with genetically male sterile (GMS) plants to generate heterozygous lines. In order to confirm their differences on resistance, the parental and generated lines were inoculated with 9 P. brassicae field isolates in the greenhouse and resistant test also evaluated in different naturally infested fields. The CR pyramiding lines exhibited high levels of resistance to the most isolates than line containing any single resistance gene; comparatively, the homozygous lines demonstrated a higher resistance than the heterozygous. The genetic variation among P. brassicae isolates was analyzed through simple sequence repeat (SSR) marker and significant divergences were recorded among the 9 tested field isolates and the result was consistent with data acquired from the greenhouse and natural field experiments. This study will certainly provide a novel molecular strategy for the breeding of the durable CR B. napus varieties in future breeding programs.
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