Integrated metabolomic and proteomics profiling reveals the promotion of Lactobacillus reuteri LR1 on amino acid metabolism in the gut-liver axis of weaned pigs
文献类型： 外文期刊
作者： Yi, Hongbo 1 ; Yang, Guangda 1 ; Xiong, Yunxia 1 ; Wu, Qiwen 1 ; Xiao, Hao 1 ; Wen, Xiaolu 1 ; Yang, Xuefen 1 ; Wang, Li; 1 ;
作者机构： 1.Guangdong Acad Agr Sci, State Key Lab Livestock & Poultry Breeding,Inst A, Key Lab Anim Nutr & Feed Sci South China,Minist A, Guangdong Publ Lab Anim Breeding & Nutr,Guangdong, 1 Dafeng 1st St, Guangzhou 510640, Guangdong, Peoples R China
期刊名称：FOOD & FUNCTION （ 影响因子：5.396； 五年影响因子：5.534 ）
ISSN： 2042-6496
年卷期： 2019 年 10 卷 11 期
页码：
收录情况： SCI
摘要： Lactobacillus reuteri LR1 improved growth performance of weaned pigs in our previous study. The objective of this study was to reveal effects of L. reuteri LR1 on amino acid (AA) metabolism in weaned pigs and its underlying mechanism using metabolomic and proteomics methods. Weaned pigs were fed a basal diet (CON) or the basal diet supplemented with 5 x 10(10) cfu kg(-1)L. reuteri LR1 (LR1) for a 14 d period. Untargeted metabolomic analysis of the liver showed that LR1 up-regulated 33 metabolites and most of them were related to AA metabolism. Quantitative proteomics found that differential proteins were mainly involved in a metabolic process in the ileal mucosa of LR1 vs. CON. Integrated metabolomic and proteomics analysis showed that the LR1's enhancement of AA metabolism in the gut-liver axis is mediated by the up-regulated intestinal AA transporters in the pathway of protein digestion and absorption. Moreover, qPCR results confirmed that LR1 increased (p < 0.05) mRNA abundances of AA transporters (PepT1, EAAT3, rBAT, B(0)AT1, and b(0,+)AT) in the ileal mucosa compared with CON. Furthermore, western blot analysis showed that LR1 activated the mammalian target of the rapamycin complex 1 (mTORC1) signaling pathway by increasing the phosphorylation of S6 and 70S6K1 in the gut-liver axis of weaned pigs. Together, these data indicated that dietary supplemented LR1 enhanced AA metabolism by up-regulating intestinal AA transporter expression and activating the mTORC1 signaling pathway in the gut-liver axis of weaned pigs.
- 相关文献
作者其他论文 更多>>
-
Effect of mixed meal replacement of soybean meal on growth performance, nutrient apparent digestibility, and gut microbiota of finishing pigs
作者：He, Zhentao;Liu, Shuai;Wen, Xiaolu;Cao, Shuting;Zhan, Xianliang;Hou, Lei;Li, Yaojie;Chen, Shaozhen;Zheng, Huayu;Deng, Dongyan;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wang, Li
关键词：rapeseed meal; cotton meal; sunflower meal; finishing pigs; short-chain fatty acid
-
Effect of Miscellaneous Meals Replacing Soybean Meal in Feed on Growth Performance, Serum Biochemical Parameters, and Microbiota Composition of 25-50 kg Growing Pigs
作者：Zhan, Xianliang;Hou, Lei;He, Zhentao;Cao, Shuting;Wen, Xiaolu;Liu, Shuai;Li, Yaojie;Chen, Shaozhen;Zheng, Huayu;Deng, Dongyan;Gao, Kaiguo;Yang, Xuefen;Jiang, Zongyong;Wang, Li
关键词：miscellaneous meals; soybean meal; growth performance; microbiota
-
Accurate models and nutritional strategies for specific oxidative stress factors: Does the dose matter in swine production?
作者：Hong, Changming;Huang, Yujian;Cao, Shuting;Wang, Li;Yang, Xuefen;Hu, Shenglan;Gao, Kaiguo;Jiang, Zongyong;Xiao, Hao
关键词：Accurate models; Dose; Nutritional strategies; Oxidative stress; Swine
-
Maternal resveratrol improves the intestinal health and weight gain of suckling piglets during high summer temperatures: The involvement of exosome-derived microRNAs and immunoglobin in colostrum
作者：Hong, Changming;Huang, Yujian;Wen, Xiaolu;Wang, Li;Yang, Xuefen;Gao, Kaiguo;Jiang, Zongyong;Xiao, Hao;Hong, Changming;Huang, Yujian;Wen, Xiaolu;Wang, Li;Yang, Xuefen;Gao, Kaiguo;Jiang, Zongyong;Xiao, Hao;Hong, Changming;Yang, Guan
关键词：Colostrum; Exosome; Immunoglobulin; Intestinal health; Resveratrol
-
Fermented Corn-Soybean Meal Improved Growth Performance and Reduced Diarrhea Incidence by Modulating Intestinal Barrier Function and Gut Microbiota in Weaned Piglets
作者：Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong;Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong;Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong;Qiu, Yueqin;Tang, Jiaxi;Wang, Li;Yang, Xuefen;Jiang, Zongyong
关键词：fermented mixed feed; weaned piglets; growth performance; intestinal barrier; butyrate; gut microbiota
-
Characterization of a novel gammapartitivirus infecting the phytopathogenic fungus Pyricularia oryzae
作者：Fan, Yu;Zhao, Wenhua;Tang, Xiaolin;Wang, Li;Yang, Mei;Zhou, Erxun;He, Zhenrui;Yang, Yingqing;Cheng, Baoping
关键词：
-
Dietary supplementation with Neolamarckia cadamba leaf extract improves broiler meat quality by enhancing antioxidant capacity and regulating metabolites
作者：Wang, Cheng;Wang, Li;Wang, Cheng;Chen, Dandan;Wu, Shou;Zhou, Wei;Chen, Xiaoyang;Zhang, Qing
关键词：Neolamarckia cadamba leaf extract; Broiler; Meat quality; Antioxidant capacity; Metabolomics
