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Proteomic Landscape Has Revealed Small Rubber Particles Are Crucial Rubber Biosynthetic Machines for Ethylene-Stimulation in Natural Rubber Production

文献类型： 外文期刊

作者： Wang, Dan 1 ; Xie, Quanliang 1 ; Sun, Yong 4 ; Tong, Zheng 2 ; Chang, Lili 2 ; Yu, Li 1 ; Zhang, Xueyan 1 ; Yuan, Boxuan; 1 ;

作者机构： 1.Hainan Normal Univ, Coll Life Sci, Key Lab Ecol Trop Isl, Minist Educ, Haikou 571158, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

3.Shihezi Univ, Coll Life Sci, Minist Educ, Key Lab Xinjiang Phytomed Resource & Utilizat, Shihezi 832003, Peoples R China

4.Chinese Acad Trop Agr Sci, Rubber Res Inst, Danzhou 571737, Peoples R China

5.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

6.CIRAD, AGAP, UMR, F-34398 Montpellier, France

关键词： Hevea brasiliensis; natural rubber biosynthesis; protein phosphorylation; quantitative proteomics; small rubber particle

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2019 年 20 卷 20 期

页码：

收录情况： SCI

摘要： Rubber particles are a specific organelle for natural rubber biosynthesis (NRB) and storage. Ethylene can significantly improve rubber latex production by increasing the generation of small rubber particles (SRPs), regulating protein accumulation, and activating many enzyme activities. We conducted a quantitative proteomics study of different SRPs upon ethylene stimulation by differential in-gel electrophoresis (DIGE) and using isobaric tags for relative and absolute quantification (iTRAQ) methods. In DIGE, 79 differentially accumulated proteins (DAPs) were determined as ethylene responsive proteins. Our results show that the abundance of many NRB-related proteins has been sharply induced upon ethylene stimulation. Among them, 23 proteins were identified as rubber elongation factor (REF) and small rubber particle protein (SRPP) family members, including 16 REF and 7 SRPP isoforms. Then, 138 unique phosphorylated peptides, containing 129 phosphorylated amino acids from the 64 REF/SRPP family members, were identified, and most serine and threonine were phosphorylated. Furthermore, we identified 226 DAPs from more than 2000 SRP proteins by iTRAQ. Integrative analysis revealed that almost all NRB-related proteins can be detected in SRPs, and many proteins are positively responsive to ethylene stimulation. These results indicate that ethylene may stimulate latex production by regulating the accumulation of some key proteins. The phosphorylation modification of REF and SRPP isoforms might be crucial for NRB, and SRP may act as a complex natural rubber biosynthetic machine.

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