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Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

文献类型： 外文期刊

作者： Yarra, Rajesh 1 ; Jin, Longfei 1 ; Zhao, Zhihao 1 ; Cao, Hongxing 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Peoples R China

2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

关键词： in vitro propagation; Agrobacterium; particle bombardment; CRISPR; Cas9

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2019 年 20 卷 21 期

页码：

收录情况： SCI

摘要： Oil palm (Elaeis guineensis, Jacq.) is a prominent vegetable-oil-yielding crop. Cultivating high-yielding oil palm with improved traits is a pre-requisite to meet the increasing demands of palm oil consumption. However, tissue culture and biotechnological approaches can resolve these concerns. Over the past three decades, significant research has been carried out to develop tissue culture and genetic transformation protocols for oil palm. Somatic embryogenesis is an efficient platform for the micropropagation of oil palm on a large scale. In addition, various genetic transformation techniques, including microprojectile bombardment, Agrobacterium tumefaciens mediated, Polyethylene glycol mediated mediated, and DNA microinjection, have been developed by optimizing various parameters for the efficient genetic transformation of oil palm. This review mainly emphasizes the methods established for in vitro propagation and genetic transformation of oil palm. Finally, we propose the application of the genome editing tool CRISPR/Cas9 to improve the various traits in this oil yielding crop.

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