Fluorometric aptasensor for cadmium(II) by using an aptamer-imprinted polymer as the recognition element
文献类型： 外文期刊
作者： Li, Shuhuai 1 ; Ma, Xionghui 1 ; Pang, Chaohai 1 ; Tian, Hai 1 ; Xu, Zhi 1 ; Yang, Yan 4 ; Lv, Daizhu 1 ; Ge, Huilin 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Anal & Test Ctr, Haikou 571101, Hainan, Peoples R China
2.Hainan Prov Key Lab Qual & Safety Trop Fruits & V, Haikou 571101, Hainan, Peoples R China
3.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
4.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Hainan, Peoples R China
关键词： Fluorescence spectra; UV-visible spectroscopy; Cyclic voltammetry; Transmission electron microscopy; X-ray photoelectron spectroscopy
期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）
ISSN： 0026-3672
年卷期： 2019 年 186 卷 12 期
页码：
收录情况： SCI
摘要： A fluorometric assay is described for the determination of Cd(II) in environmental and agricultural samples. It is making use of a molecularly imprinted polymer (MIP) and aptamer as dual recognition units, while carbon quantum dots (co-doped with sulphur and nitrogen) and gold nanoparticles (SN-CQD/Au) act as the fluorophores. The aptamer-modified MIP was placed on an SN-CQD/Au-modified indium tin oxide glass electrode. Cd(II) was detected with high selectivity by the recognition sites of the aptamer in the MIP. Fluorescence, with excitation/emission peaks at 370/430 nm, is quenched by Cd(II). Response is linear in the 20 pM to 12 nM concentration range. The detection limit is 1.2 pM. The sensor is selective for Cd(II), and recoveries from spiked waters, soils and vegetables real-world samples range between 82.1 and 113.9%.
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