文献类型： 外文期刊

作者： Li, Y. X. ¹ ; Feng, X. P. ¹ ; Wang, H. L. ¹ ; Meng, C. H. ¹ ; Zhang, J. ¹ ; Qian, Y. ¹ ; Zhong, J. F. ¹ ; Cao, S. X. ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Prov Platform Conservat & Utilizat Agr Ge, Nanjing 210014, Jiangsu, Peoples R China

3.Minist Agr, Key Lab Crop & Anim Integrated Farming, Nanjing 210014, Jiangsu, Peoples R China

4.Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China

关键词： Hu sheep; Heat stress; Transcriptome sequencing; GO annotation; KEGG pathway

期刊名称：CELL STRESS & CHAPERONES （ 影响因子：3.667； 五年影响因子：3.717 ）

ISSN： 1355-8145

年卷期： 2019 年 24 卷 6 期

页码：

收录情况： SCI

摘要： Heat stress (HS) seriously affects animal performance. In view of global warming, it is essential to understand the regulatory mechanisms by which animals adapt to heat stress. In this study, our aim was to explore the genes and pathways involved in heat stress in sheep. To this end, we used transcriptome analysis to understand the molecular responses to heat stress and thereby identify means to protect sheep from heat shock. To obtain an overview of the effects of heat stress on sheep, we used the hypothalamus for transcriptome sequencing and identified differentially expressed genes (DEGs; false discovery rate (FDR) < 0.01; fold change > 2) during heat stress. A total of 1423 DEGs (1122 upregulated and 301 downregulated) were identified and classified into Gene Ontology (GO) categories and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Heat stress triggered dramatic and complex alterations in gene expression in the hypothalamus. We hypothesized that heat stress induced apoptosis and dysfunction in cells and vital organs and affected growth, development, reproduction, and circadian entrainment via the calcium signaling pathway, which influences ribosome assembly and function. Real-time PCR was used to evaluate the expression of the genes regulating important biological functions or whose expression profiles were significantly changed after acute heat stress (FDR < 0.01; fold change > 4), and the results showed that the expression patterns of these genes were consistent with the results of transcriptome sequencing, indicating that the credibility of the sequencing results. Our data indicated that heat stress induced calcium dyshomeostasis, blocked biogenesis, caused ROS accumulation, impaired the antioxidant system and innate defense, and induced apoptosis through the P53 signaling pathway activated by PEG3, decreased growth and development, and enhanced organ damage. These data is very important and helpful to elucidate the molecular mechanism of heat stress and finally to find ways to deal with heat stress damage in sheep.