文献类型： 外文期刊
作者： Luan, Aiping 1 ; He, Yehua 2 ; Xie, Tao 2 ; Chen, Chengjie 2 ; Mao, Qi 2 ; Wang, Xiaoshuang 2 ; Li, Chuhao 2 ; Ding, Yaqi 2 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Hainan, Peoples R China
2.South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
3.Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China
关键词： pineapple; AcSERK1; embryonic cell-specific promoter; regulatory sequences; somatic embryogenesis
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN：
年卷期： 2019 年 10 卷 11 期
页码：
收录情况： SCI
摘要： Plant tissue culture methods, such as somatic embryogenesis, are attractive alternatives to traditional breeding methods for plant propagation. However, they often suffer from limited efficiency. Somatic embryogenesis receptor kinase (SERK)1 is a marker gene of early somatic embryogenesis in several plants, including pineapple. It can be selectively induced and promotes a key step in somatic embryogenesis. We investigated the embryonic cell-specific transcriptional regulation of AcSERK1 by constructing a series of vectors carrying the GUS (Beta-glucuronidase) reporter gene under the control of different candidate cis-regulatory sequences. These vectors were transfected into both embryonic and non-embryonic callus, and three immature embryo stages and the embryonic-specific activity of the promoter fragments was analyzed. We found that the activity of the regulatory sequence of AcSERK1 lacking -983 nt similar to-880 nt, which included the transcription initiation site, was significantly reduced in the embryonic callus of pineapple, accompanied by the loss of embryonic cell-specific promoter activity. Thus, this fragment is an essential functional segment with highly specific promoter activity for embryonic cells, and it is active only from the early stages of somatic embryo development to the globular embryo stage. This study lays the foundation for identifying mechanisms that enhance the efficiency of somatic embryogenesis in pineapple and other plants.
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