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Development of disk-shaped monolithic microplates for detecting multiple mycotoxins

文献类型： 外文期刊

作者： Wang, He-Ye 3 ; Yang, Ya-Li 1 ; Wang, Yue 1 ; Zhang, Shuai-Feng 1 ; Li, Li 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Facil & Equipment, Nanjing 210014, Jiangsu, Peoples R China

2.Minist Agr, Key Lab Protected Agr Engn Middle & Lower Reaches, Nanjing 210014, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, State Key Lab Cultivat Base MOST, Jiangsu Key Lab Food Qual & Safety, Nanjing 210014, Jiangsu, Peoples R China

4.Jiangsu Univ, Sch Mat Sci & Engn, Zhenjiang, Jiangsu, Peoples R China

期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

ISSN： 1759-9660

年卷期： 2019 年 11 卷 48 期

页码：

收录情况： SCI

摘要： Organic polymer monoliths have been used extensively for separation and purification of molecules because of their excellent mass transfer properties and versatile surface modification. However, organic polymer monolithic materials have significant UV absorption, which blocked UV-visible absorbance detection. Thus, we developed novel disk-shaped mycotoxin immune-affinity monolithic microplates for quantitative detection of mycotoxins. The concentric disk-shaped format allows the obscure organic monoliths to capture mycotoxins and unobstructed UV-visible absorbance measurement was performed in the same microplates. The limits of detection of 96 disk-shaped mycotoxin immune-affinity monolithic microplates were 161.73, 22.52, 0.14, and 0.21 mu g kg(-1) for Deoxynivalenol (DON), Zearalenone (ZEN), Aflatoxin B1 (AFB1), and Ochratoxin A (OTA), respectively, which meet well the requirements for detection of foodstuffs. The recovery rate ranges of the four mycotoxins were 87-92%, 82-89%, 78-116%, and 83-87% for DON, ZEN, AFB1, and OTA disk-shaped microplates, respectively, which proved that the disk-shaped monolithic microplates have good reproducibility for mycotoxin detection. In summary, 96 disk-shaped mycotoxin immune-affinity monolithic microplates are a sensitive, accurate, stable, and convenient platform for detection of mycotoxins in agro-food.

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