Cloning and Characterization of a gene Encoding True D-cysteine Desulfhydrase from Oryza sativa
文献类型： 外文期刊
作者： Zhou, Heng 1 ; Guan, Wenxue 1 ; Zhou, Mingjian 2 ; Shen, Jie 2 ; Liu, Xin 3 ; Wu, Deliang 1 ; Yin, Xianchao 1 ; Xie, Yanj 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety,Inst Food Safe, State Key Lab Cultivat Base,Minist Agr & Rural Af, Minist Sci & Technol,Key Lab Control Technol & St, Nanjing 210014, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Coll Life Sci, Lab Ctr Life Sci, Nanjing 210095, Jiangsu, Peoples R China
3.Qingdao Agr Univ, Key Lab Plant Biotechnol Univ Shandong Prov, Life Sci Coll, Qingdao 266109, Shandong, Peoples R China
关键词： D-cysteine desulfhydrase; Hydrogen sulfide; 1-aminocyclopropane-1-carboxylate deaminase; Subcellular localization
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）
ISSN： 0735-9640
年卷期： 2020 年 38 卷 1 期
页码：
收录情况： SCI
摘要： Hydrogen sulfide (H2S) has been regarded as the third gasotransmitter and plays an active role in multiple signaling events of plants and animals. Cysteine desulfhydrases (CDes), including both D- and L-cysteine desulfhydrases (D/L-CDes) that degrade L- or D-cysteine into H2S, pyruvate, and ammonium, are considered the key enzymes responsible for endogenous H2S generation in plants. Several D-CDes are homologous to 1-aminocyclopropane-1-carboxylate deaminase (ACCD) and possess both ACCD and D-CDes activities, thus not a real specific D-CDes. However, little attention had been paid to true D-CDes and little information has been known about this protein in plants. In this study, a putative D-CDes transcript was cloned and characterized from Oryza sativa which encodes a protein with 423 amino acids possessing D-CDes activity and named as OsDCD1. Neither activities of ACCD nor O-acetyl-L-serine (thiol) lyase (OASTL) can be detected from OsDCD1 recombinant protein. For D-Cys, the K-m of OsDCD1 is 0.13 +/- 0.01 mM and the V-m is 111.55 +/- 1.91 units mg(-1) of protein. The pH-optimum and temperature-optimum of the OsDCD1 are 8.5 and 35 degrees C, respectively. By site-directed mutagenesis, mutation of S357E or S357E/T589L almost fully abolished the D-CDes activity of OsDCD1, while the T389L mutant retained only partial D-CDes activity by 3.7%, indicating these two amino acid residues play critical roles for the maintenance of OsDCD1 activity. Besides, subcellular localization analysis in rice protoplast revealed that the OsDCD1 localizes in the chloroplast but not mitochondria, which is different from DCD1 in Arabidopsis. The qRT-PCR analysis further showed that the abundance of OsDCD1 transcript was widely regulated by different hormones and chemical reagents we used. In general, our results provided evidence that OsDCD1 is a potentially important endogenous H2S producing enzyme in rice, which may play an important role in plant growth regulators and chemical stimuli.
- 相关文献
作者其他论文 更多>>
-
Fusarium graminearum rapid alkalinization factor peptide negatively regulates plant immunity and cell growth via the FERONIA receptor kinase
作者：Wang, Yujie;Liu, Xin;Yuan, Bingqin;Chen, Xue;Zhao, Hanxi;Ali, Qurban;Zheng, Minghong;Tan, Zheng;Yao, Hemin;Zheng, Shuqing;Wu, Jingni;Xu, Jianhong;Shi, Jianrong;Wu, Huijun;Gao, Xuewen;Gu, Qin;Liu, Xin;Xu, Jianhong;Shi, Jianrong
关键词：Fusarium graminearum; rapid alkalinization factor; receptor kinase FERONIA; host immunity; plant growth
-
Twin S-Scheme g-C3N4/CuFe2O4/ZnIn2S4 Heterojunction with a Self-Supporting Three-Phase System for Photocatalytic CO2 Reduction: Mechanism Insight and DFT Calculations
作者：Liu, Dongdong;Jiang, Lipeng;Chen, Dengqian;Hao, Zhengkai;Deng, Bowen;Chen, Limei;Sun, Yunyun;Liu, Huitao;Liu, Xin;Jia, Boyin
关键词：g-C3N4/CuFe2O4/ZnIn2S4 photocatalyst; twin S-scheme heterojunction; self-supporting three-phase system; photocatalytic CO2 reduction; DFT calculations
-
High selectivity and efficient utilization of H2O2 in a metal-free Electro-Fenton system with nitrogen doped cathode for the degradation of sulfamethazine
作者：Liu, Dongdong;Chen, Dengqian;Jiang, Lipeng;Hao, Zhengkai;Deng, Bowen;Chen, Limei;Jia, Boyin;Sun, Yunyun;Liu, Huitao;Liu, Xin
关键词：metal-free Electro-Fenton system; nitrogen doped cathode; H(2)O(2)utilization; SMZ degradation; DFT calculation
-
Photocatalytic self-Fenton degradation of ciprofloxacin over S-scheme CuFe2O4/ZnIn2S4 heterojunction: Mechanism insight, degradation pathways and DFT calculations
作者：Liu, Dongdong;Jiang, Lipeng;Chen, Dengqian;Hao, Zhengkai;Deng, Bowen;Chen, Limei;Sun, Yunyun;Liu, Huitao;Liu, Xin;Jia, Boyin
关键词：CuFe2O4/ZnIn2S4 photocatalyst; S-scheme heterojunction; Photocatalytic self-Fenton system; CIP degradation; DFT calculations
-
Distribution and effects of microplastics as carriers of heavy metals in river surface sediments
作者：Wang, Changyuan;Liu, Xin;Ma, Qianqian;Xing, Shuyu;Yuan, Lubin;Ma, Yan;Wang, Changyuan;Ma, Yan;Wang, Changyuan;Ma, Yan
关键词：Season; River; Abundance; Microplastics; Metal; Sediment
-
Metconazole inhibits fungal growth and toxin production in major Fusarium species that cause rice panicle blight
作者：Wang, Bingbing;Wang, Shuang;Qiu, Jianbo;Gao, Tao;Shi, Jianrong;Xu, Jianhong;Liu, Xin;Wang, Bingbing;Wang, Shuang;He, Dan;Zhou, Yunyun;Qiu, Jianbo;Lee, Yin-Won;Shi, Jianrong;Xu, Jianhong;Liu, Xin;He, Dan;Zhou, Yunyun;Xu, Jianhong;Liu, Xin;Lee, Yin-Won
关键词：Rice panicle blight; Toxigenic Fusarium spp.; Metconazole; Ergosterol; Control efficacy
-
Effect of Crop Rotation on Fusarium Mycotoxins and Fusarium Species in Cereals in Sichuan Province (China)
作者：Dong, Fei;Wu, Deliang;Shen, Guanghui;Liu, Xin;Xu, Jian Hong;Shi, Jian Rong;Dong, Fei;Mokoena, Mduduzi P.;Olaniran, Ademola O.;Shi, Jian Rong;Chen, Xiangxiang;Liu, Xin;Xu, Jian Hong;Shi, Jian Rong;Lei, Xinyu;Zhang, Yifan;Li, Ying;Lee, Yin-Won
关键词：crop rotation; Fusarium; Fusarium species; pathogenicity
