A macromolecular alpha-glucan from fruiting bodies of Volvariella volvacea activating RAW264. 7 macrophages through MAPKs pathway
文献类型： 外文期刊
作者： Cui, FengJie 1 ; Jiang, Lihua 1 ; Qian, LiShun 1 ; Sun, WenJing 1 ; Tao, TingLei 1 ; Zan, Xinyi 1 ; Yang, Yan 2 ; Wu, Di; 1 ;
作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
2.Shanghai Acad Agr Sci, Natl Engn Res Ctr Edible Fungi, Shanghai 201403, Peoples R China
3.Jiangxi Prov Engn & Technol Ctr Food Addit Biopro, Dexing 334221, Peoples R China
4.Jiangsu Jiangnan Biotech Co Ltd, Danyang 212300, Jiangsu, Peoples R China
关键词： Volvariella volvacea; alpha-Glucan; Structural analysis; Immunomodulatory activity; MAPKs pathways
期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）
ISSN： 0144-8617
年卷期： 2020 年 230 卷
页码：
收录情况： SCI
摘要： A novel macromolecular polysaccharide VGPI-a was purified from Volvariella volvacea fruiting bodies with ultrasound-assisted extraction, ion exchange and gel chromatography. VGPI-a was proved as a alpha- glucan with Mw of 1435.6 kDa and contained a 1,4-linked D-Glcp backbone with the substitution at C-6 with 1-linked D-Glcp residue. Congo-red test, AFM and SEM analysis showed VGPI-a had a triple-helical conformation and the interacted chains to form a small screw-rod and dispersed appearance. VGPI-a had no cytotoxic effect on macrophage RAW264.7 cells in vitro and significantly enhanced the production and mRNA expression of NO, TNF-alpha, IL-6 and IL-1 beta in a dose-dependent manner. Further analyses demonstrated that VGPI-a activated the MAPK signaling pathway by improving the phosphorylated levels of p38, JNK and ERK in RAW264.7 cells to promote the expression and secretion of above cytokines. These findings would provide a better understanding of V. volvacea glucan and its potential immunomodulating mechanisms.
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