Genome-wide identification and analysis of genes encoding cuticular proteins in the endoparasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae)
文献类型： 外文期刊
作者： Wang, Jiale 1 ; Jin, Hongxia 1 ; Yang, Lei 1 ; Ye, Xinhai 1 ; Xiao, Shan 1 ; Song, Qisheng 3 ; Stanley, David 4 ; Ye, Gon 1 ;
作者机构： 1.Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China
2.Zhejiang Univ, Inst Insect Sci, Key Lab Mol Biol Crop Pathogens & Insects, Minist Agr & Rural Affairs, Hangzhou, Zhejiang, Peoples R China
3.Univ Missouri, Div Plant Sci, Columbia, MO USA
4.USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA
5.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou, Zhejiang, Peoples R China
关键词： apidermin; cuticular proteins; expression profile; hydrophobicity; Pteromalus puparum
期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）
ISSN： 0739-4462
年卷期： 2020 年 103 卷 2 期
页码：
收录情况： SCI
摘要： The multifunctional insect cuticle serves as the exoskeleton, determines body shape, restricts water loss, provides attachment sites for muscles and internal organs and is a formidable barrier to invaders. It is morphologically divided into three layers, including envelope, epicuticle, and procuticle and is composed of chitin and cuticular proteins (CPs). Annotation of CPs and their cognate genes may help understand the structure and functions of insect cuticles. In this paper, we interrogated the genome of Pteromalus puparum, an endoparasitoid wasp that parasitizes Pieris rapae and Papilio xuthus pupae, and identified 82 genes encoding CPs belonging to six CP families, including 62 in the CPR family, 8 in CPAP3, 5 in CPF/CPFL, 2 low complexity proteins, 2 in TWDL, and 3 in Apidermin. We used six RNA-seq libraries to determine CP gene expression profiles through development and compared the cuticle hydrophobicity between the P. puparum and the ectoparasitoid Nasonia vitripennis based on GRAVY values of CPR sequences. In the Nasonia-Pteromalus comparison, we found in both N. vitripennis and P. puparum, the peak of their CPR hydrophobicity displayed at their pupal stage, whereas their adult stage showed the lowest level. Except at the adult stage, the CPR hydrophobicity in N. vitripennis is always higher than P. puparum. Finally, we identified three novel Apidermin genes, a family found solely in Hymenoptera and revealed a new sequence feature of this family. This new information contributes to a broader understanding of insect CPs generally.
- 相关文献
作者其他论文 更多>>
-
A PAM-Free One-Step Asymmetric RPA and CRISPR/Cas12b Combined Assay (OAR-CRISPR) for Rapid and Ultrasensitive DNA Detection
作者：Yang, Lei;Chen, Guanwei;Wei, Wei;Peng, Cheng;Ding, Lin;Chen, Xiaoyun;Xu, Xiaoli;Wang, Xiaofu;Xu, Junfeng;Chen, Guanwei;Wu, Jian
关键词：
-
Combined impacts of microplastics and cadmium on the liver function, immune response, and intestinal microbiota of crucian carp (Carassius carassius)
作者：Wei, Wei;Yang, Qiufeng;Xiang, Dan;Chen, Xiaoyun;Wen, Zhengrong;Wang, Xiaofu;Xu, Xiaoli;Peng, Cheng;Yang, Lei;Xu, Junfeng;Yang, Qiufeng;Xiang, Dan;Wen, Zhengrong;Luo, Mingzhong
关键词：Microplastics; Cadmium; Liver function; Immune response; Intestinal microbiota
-
Identification and gene expression analysis of serine proteases and their homologs in the Asian corn borer Ostrinia furnacalis
作者：Yang, Lei;Xu, Xiaoli;Wei, Wei;Chen, Xiaoyun;Peng, Cheng;Wang, Xiaofu;Xu, Junfeng
关键词：
-
Toll and IMD Immune Pathways Are Important Antifungal Defense Components in a Pupal Parasitoid, Pteromalus puparum
作者：Yang, Lei;Yang, Lei;Wang, Xiaofu;Peng, Cheng;Chen, Xiaoyun;Xu, Xiaoli;Xu, Junfeng;Wei, Wei;Ye, Gongyin;Yang, Lei;Yang, Lei;Wei, Wei;Ye, Gongyin
关键词：parasitoid; Toll pathway; IMD pathway; entomopathogenic fungi; scanning electron microscopy (SEM)
-
Genome-wide analysis of ALDH gene family in jujube and identification of ZjALDH3F3 for its important role in high-temperature tolerance
作者：Li, Lili;Yang, Lei;Fan, Dingyu;Jin, Juan;Xiaokelaiti, Subina;Hao, Qing;He, Yanjun
关键词：Overexpression; Heat tolerance; Promoter; Jujube
-
Altered Gene Expression of the Parasitoid Pteromalus puparum after Entomopathogenic Fungus Beauveria bassiana Infection
作者：Li, Jinting;Wang, Xiaofu;Xu, Xiaoli;Xu, Junfeng;Yang, Lei;Yang, Lei;Xiao, Shan;Xiong, Shijiao;Ye, Gongyin;Yang, Lei;Yang, Lei;Xiao, Shan;Xiong, Shijiao;Ye, Gongyin
关键词：fungi; infection; survival rate; host responses; transcriptome analysis
-
Effects of non-lethal Cry1F toxin exposure on the growth, immune response, and intestinal microbiota of silkworm (Bombyx mori)
作者：Xu, Xiaoli;Liang, Xiaowei;Wei, Wei;Peng, Cheng;Wang, Xiaofu;Chen, Xiaoyun;Yang, Lei;Xu, Junfeng;Liang, Xiaowei;Ding, Xiaohao
关键词：Cry1F toxin; Silkworm; Enzyme activity; Immune response; Intestinal microbiota
