Identification of a linear B-cell epitope on glycoprotein (GP) 2a of porcine reproductive and respiratory syndrome virus (PRRSV)
文献类型: 外文期刊
作者: Shi, Xibao 1 ; Fan, Xiaomin 1 ; Nie, Shouyi 2 ; Kou, Liyun 2 ; Zhang, Xiaozhuan 1 ; Liu, Hongliang 2 ; Ji, Shengdong 1 ;
作者机构: 1.Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China
2.Zhengzhou Univ, Dept Bioengn, Zhengzhou 450000, Henan, Peoples R China
3.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Henan, Peoples R China
4.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China
关键词: PRRSV; GP2a; Epitope
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN: 0141-8130
年卷期: 2019 年 139 卷
页码:
收录情况: SCI
摘要: Glycoprotein (GP) 2a was a minor structural protein of porcine reproductive and respiratory syndrome virus (PRRSV) and was one of crucial proteins for PRRSV to bind cell receptor, which indicated that there were neutralizing epitopes on GP2a. In the present work, we used mouse anti-GP2a(41-208aa) serum and one GP2a(41-208aa) specific monoclonal antibody (McAb) to identify B-cell epitopes of GP2a by peptide-based ELISA. A liner B-cell epitope F(194)PTPGSRPKLHDFQQ(208) was identified. However, the results of virus neutralization experiment showed that the McAb could not reduce the titers of PRRSV, which indicated that the identified epitope was not the neutralizing epitope of PRRSV. While the amino acid sequence of this epitope was conserved in North American (type 2) PRRSV, which suggested that this epitope might be diagnostic potential for type 2 PRRSV strains. In conclusion, our present work identified a new epitope on GP2a and this epitope might be diagnostic potential for type 2 PRRSV strains. (c) 2019 Published by Elsevier B.V.
- 相关文献
作者其他论文 更多>>
-
Enhancing humoral and mucosal immune response of PED vaccine candidate by fusing S1 protein to nanoparticle multimerization
作者:Li, Minghui;Sun, Xueke;Wang, Siqiao;Wang, Yanan;Wang, Yue;Zhang, Gaiping;Li, Minghui;Sun, Xueke;Chen, Yilan;Wang, Siqiao;Li, Qin;Wang, Yanan;Wang, Yue;Li, Ruiqi;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Ding, Peiyang;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词:PEDV; S1 protein; Nanoparticle; Subunit vaccine; Mucosal immunity
-
Pseudorabies virus tegument protein US2 antagonizes antiviral innate immunity by targeting cGAS-STING signaling pathway
作者:Kong, Zhengjie;Zhang, Yifeng;Guan, Kaifeng;Yao, Wanzi;Kang, Yu;Lu, Xinyi;Zhang, Gaiping;Chen, Xing;Gong, Lele;Wang, Lele;Zhang, Yuhang;Du, Yongkun;Sun, Aijun;Zhuang, Guoqing;Wan, Bo;Zhang, Gaiping;Zhao, Jianguo;Zhang, Gaiping;Zhang, Gaiping
关键词:pseudorabies virus; cGAS-STING; tegument protein US2; TRIM21; immune invasion
-
IFI16 Positively Regulates RIG-I-Mediated Type I Interferon Production in a STING-Independent Manner
作者:Shi, Xibao;Wei, Menglu;Feng, Yuwen;Yang, Yuanhao;Zhang, Xiaozhuan;Chen, Hao;Xing, Yuqi;Wang, Keqi;Wang, Wensheng;Wang, Li;Zhang, Gaiping;Wang, Aiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Shi, Xibao;Zhang, Gaiping
关键词:IFN-gamma-inducible protein 16; interferon gene-stimulating protein; retinoic acid-inducible gene I; type I interferon
-
A universal design of restructured dimer antigens: Development of a superior vaccine against the paramyxovirus in transgenic rice
作者:Ma, Fanshu;Zhang, Longxian;Zhang, Erqin;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Zhang, Gaiping;Ma, Fanshu;Xu, Qianru;Yang, Daichang;Li, Qingmei;Guo, Junqing;Li, Rui;Wang, Li;Wang, Yanan;Zhao, Xiangyue;Chai, Shujun;Yin, Heng;Li, Kunpeng;Rao, Zihe;Rao, Zihe;Rao, Zihe
关键词:structural vaccine; transgenic rice; BCR; paramyxovirus
-
Double-layered N-S1 protein nanoparticle immunization elicits robust cellular immune and broad antibody responses against SARS-CoV-2
作者:Li, Ruiqi;Chang, Zejie;Cheng, Anchun;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Zhang, Gaiping;Li, Ruiqi;Chang, Zejie;Wang, Yanan;Li, Minghui;Chen, Yilan;Fan, Lu;Wang, Siqiao;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Chang, Zejie;Wang, Yanan;Li, Minghui;Sun, Xueke;Liu, Siyuan;Zhang, Gaiping;Liu, Hongliang;Ding, Peiyang;Zhang, Gaiping
关键词:COVID-19; Coronavirus; SARS-CoV-2; Nanoparticle; Subunit vaccine; Variants
-
Porcine reproductive and respiratory syndrome virus triggers Golgi apparatus fragmentation-mediated autophagy to facilitate viral self-replication
作者:Zhao, Shuang-shuang;Qian, Qisheng;Zhang, Gaiping;Zhao, Shuang-shuang;Qian, Qisheng;Chen, Xin-xin;Lu, Qingxia;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Zhang, Gaiping
关键词:autophagy; GA fragmentation; GRASP65; Nsp2; PRRSV; RAB2; ULK1
-
Phylogenetically evolutionary analysis provides insights into the genetic diversity and adaptive evolution of porcine deltacoronavirus
作者:Guo, Zhenhua;Lu, Qingxia;Jin, Qianyue;Xing, Guangxu;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Li, Peng
关键词:Porcine deltacoronavirus; Genetic diversity; Recombination; Adaptive evolution; Spike protein