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A rapid colloidal gold-based immunochromatographic strip assay for monitoring nitroxynil in milk

文献类型： 外文期刊

作者： Na, Guanqiong 1 ; Hu, Xiaofei 2 ; Yang, Jifei 2 ; Sun, Yaning 2 ; Kwee, Sharon 3 ; Tang, Liang 3 ; Xing, Guangxu 2 ; Xing 1 ;

作者机构： 1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

2.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Zhengzhou, Peoples R China

3.Univ Texas San Antonio, Dept Biomed Engn, San Antonio, TX USA

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou, Peoples R China

关键词： nitroxynil; monoclonal antibody; immunochromatographic strip; rapid test

期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.638； 五年影响因子：3.802 ）

ISSN： 0022-5142

年卷期： 2020 年 100 卷 5 期

页码：

收录情况： SCI

摘要： BACKGROUND Nitroxynil (NIT) is a veterinary drug against hepatic fluke disease for food-producing cattle and sheep. NIT has a long half-life time in animals since it is highly bound to plasma protein. Therefore NIT possibly remains in animal edible tissues or milk due to drug abuse. In this study, a specific murine monoclonal antibody (mAb) against NIT was prepared and an immunochromatographic strip assay based on the mAb was developed for screening NIT in milk. RESULTS The affinity constant of the anti-NIT mAb was 2.93 x 10(10) and the anti-NIT mAb had almost no cross-reactivity with other analogs, so that it showed good specificity. The cutoff value of this test strip was considered to be 50 ng mL(-1) by the naked eye. When detected by the strip reader, the half maximum inhibition concentration (IC50) of the immunoassay strip was calculated to be 5.716 ng mL(-1) and the limit of detection (LOD) was 1.146 ng mL(-1). Intra-assay recoveries from 88.80 to 97.13% were obtained, with the highest coefficient of variation (CV) at 9.01%; inter-assay recoveries ranged from 84.60 to 106.87%, with the highest CV at 9.93%. CONCLUSION The operative procedure of the proposed method can be completed within 10 min. The strip developed in this study was a practical tool for rapid semiquantitative and quantitative detection of NIT in milk. This study suggested great potential for analytically monitoring NIT in other food samples. (c) 2019 Society of Chemical Industry

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