Detection and Molecular Characterization of BCoVs Circulating in Central China Based on the Full-length Spike Gene
文献类型： 外文期刊
作者： Shi, Zhihai 1 ; Wang, Wenjia 2 ; Xu, Zhaoxue 1 ; Zhao, Mengmeng 4 ; Lan, Yali 1 ;
作者机构： 1.Henan Acad Agr Sci, Inst Anim Husb & Vet, Zhengzhou 450002, Henan, Peoples R China
2.Henan Univ Anim Husb & Econ, Coll Pharmaceut Engn, Zhengzhou 450046, Henan, Peoples R China
3.Henan Key Lab Farm Anim Breeding & Nutr Regulat, Zhengzhou 450002, Henan, Peoples R China
4.Foshan Univ, Sch Life Sci & Engn, Foshan 528225, Guangdong, Peoples R China
关键词： BCoV; Spike gene; Phylogenetic analysis; China
期刊名称：KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI （ 影响因子：0.685； 五年影响因子：0.655 ）
ISSN： 1300-6045
年卷期： 2020 年 26 卷 2 期
页码：
收录情况： SCI
摘要： Bovine coronavirus (BCoV) is prevalent throughout the world and is an important aetiological agent of diarrhoea in new-born calves. However, little is known about the genetic diversity and molecular epidemiology of BCoV in China. In this study, a total of 127 faecal samples from diarrhoeic dairy calves from nine cities in Henan Province, Central China, were collected between 2017 and 2018 and evaluated by RTPCR for the N gene. BCoV was detected in 15% (19/127) of calves. In addition, the full-length sequence of the S gene from 13 representative BCoV strains was obtained and analysed. Sequencing results for the full-length S gene showed 97.3-97.5% nucleotide (nt) identity and 96.3-96.8% amino acid (aa) identity with the classical Mebus strain. Phylogenetic analysis based on the full-length S gene showed that the BCoV strains in this study clustered with Vietnamese and Cuban BCoV strains on a large branch of the tree. These results enrich the molecular characterization of the Chinese BCoV strains and provide the first large-scale epidemiological examination of the prevalence of BCoV in diarrhoeic calves in Henan Province, Central China.
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