In vitro assessment of berberine-loaded carboxymethyl chitosan hydrogel: A promising antimicrobial candidate for S. aureus-induced bovine mastitis treatment
文献类型： 外文期刊
作者： Wang, Wenjia 1 ; Huang, Huimin 1 ; Gu, Yuetong 1 ; Li, Jiahang 1 ; Tao, Yian 1 ; Ma, Gengshao 1 ; Shi, Zhihai 2 ;
作者机构： 1.Henan Univ Anim Husb & Econ, Coll Vet Med, Zhengzhou, Peoples R China
2.Henan Acad Agr Sci, Inst Anim Husb & Vet Med, Zhengzhou, Peoples R China
期刊名称：PLOS ONE （ 影响因子：2.6； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 20 卷 6 期
页码：
收录情况： SCI
摘要： Bovine mastitis poses significant challenges to the global dairy industry, leading to substantial economic losses and public health concerns. Staphylococcus aureus, a prevalent causative agent of bovine mastitis, depends on effective adhesion and biofilm formation to establish infections. Berberine (BER), a naturally occurring phytochemical, demonstrates broad-spectrum antibacterial activity but suffers from poor bioavailability. This study developed a composite berberine-carboxymethyl chitosan/sodium alginate hydrogel to address these limitations. The hydrogel was characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction. In vitro assessments revealed that the BER hydrogel eradicated S. aureus biofilms (42% eradication at 156.26 mu g/mL), inhibited bacterial adhesion, and reduced inflammatory cytokines (IL-6 and TNF-alpha) in S. aureus-infected MAC-T cells, with compliant biosafety biocompatibility (hemolysis rate <5%) and sustained drug release (100% over 6 h), though pH-dependent release kinetics necessitate microenvironment-specific formulation refinement. In conclusion, the BER hydrogel represents a potential therapeutic candidate for S. aureus-induced bovine mastitis.
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