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Transcriptome responses in enrofloxacin-resistant strains of Aeromonas hydrophila exposed to Forsythia suspensa

文献类型： 外文期刊

作者： Sun, Jing 1 ; Huang, Xuanyun 4 ; Dong, Yaping 1 ; Jiang, Yingying 1 ; Fu, Guihong 4 ; Hu, Kun 1 ;

作者机构： 1.Shanghai Ocean Univ, Natl Pathogen Collect Ctr Aquat Anim, 999 Hucheng Huan Rd, Shanghai 201306, Peoples R China

2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

3.Minist Agr, Key Lab Freshwater Aquat Genet Resources, Shanghai 201306, Peoples R China

4.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Shanghai 200090, Peoples R China

关键词： Aeromonas hydrophila; Transcriptome; Forsythia suspensa; Resistance; Metabolic pathway

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 517 卷

页码：

收录情况： SCI

摘要： Forsythia suspensa is a traditional Chinese medicine with antibacterial and anti-inflammatory effects. In this study, the transcriptomes of enrofloxacin (ENR)-resistant strains of Aeromonas hydrophila (ATCC7966-RS) and ENR-resistant A. hydrophila treated with F. suspensa (ATCC7966-RL) were obtained from 9,647,670 and 8,352,378 raw sequence reads, respectively. After the raw sequence reads were filtered and quality checked, the results showed that 8,576,608 and 7,482,820 clean reads were obtained from the ATCC7966-RS and ATCC7966-RL groups, respectively. The average read length was 125 bp for both strains, indicating successful sequencing of the A. hydrophila transcriptome. Furthermore, 829 genes were upregulated, and 214 genes were downregulated in the ATCC7966-RL group. The enrichment results showed that the significantly expressed genes were significantly enriched in map00280, valine, leucine and isoleucine degradation, and map00071, fatty acid degradation. The biological process enrichment of GO showed that the differentially expressed genes were significantly enriched in metabolic processes, including carboxylic acid decomposition, organic acid decomposition, amino acid decomposition, decomposition of organic nitrogen compounds and fatty acid oxidation. Single nucleotide polymorphisms (SNPs) in 82 genes with missense mutations were upregulated, and 31 genes with missense mutations were downregulated in the ATCC7966-RS and ATCC7966-RL groups. This study will provide information to recognize the differential expression of genes and the specific metabolic pathways associated with the resistance of A. hydrophila after F. suspensa treatment.

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