Development of an EvaGreen based real-time RT-PCR assay for rapid detection, quantitation and diagnosis of goose calicivirus
文献类型： 外文期刊
作者： Lin, Su 1 ; Zhang, Shizhong 1 ; Wang, Shao 1 ; Xie, Kaichun 3 ; Jiang, Dandan 1 ; Xiao, Shifeng 1 ; Chen, Xiuqin 1 ; Chen 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350003, Peoples R China
2.Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Peoples R China
3.Anim Vet & Aquat Prod Bur, Nanping 353000, Peoples R China
关键词： Goose calicivirus; RNA virus; EvaGreen real-time RT-PCR; Viral detection
期刊名称：MOLECULAR AND CELLULAR PROBES （ 影响因子：2.365； 五年影响因子：2.386 ）
ISSN： 0890-8508
年卷期： 2020 年 49 卷
页码：
收录情况： SCI
摘要： An unclassified calicivirus (CV) detected in geese was recently reported and proposed as a new member of the family Caliciviridae. There is limited information about the epidemiology, etiology and detection method of goose-origin CV (GCV) to date. In this study, an EvaGreen based fluorescence quantitative real-time RT-PCR assay was developed and optimized for the detection of GCVs. The assay sensitively detected GCV RNA template with a good linear standard curve. We also demonstrated the specificity and reproducibility of the detection method for GCVs. Thus, the method developed in this study will benefit the investigation of possible sporadic outbreaks of CV infections in geese, as well as epidemiological and etiological studies of GCVs.
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