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Transcriptomic analysis of the interactions between the Spodoptera exigua midgut and nucleopolyhedrovirus

文献类型： 外文期刊

作者： Chen, Yijuan 1 ; Guo, Ling 1 ; Wan, Nianfeng 1 ; Ji, Xiangyun 1 ; Zhang, Hao 1 ; Jiang, Jiexian 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Ecoenvironm & Plant Protect Res Inst, Ecol Control Pests Lab, 1000 Jin Qi Rd, Shanghai 201403, Peoples R China

关键词： Spodoptera exigua midgut; Nucleopolyhedrovirus; Insect-virus interaction; Peritrophic matrix (PM); Midgut immunity; Transcriptomic analysis

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2020 年 163 卷

页码：

收录情况： SCI

摘要： Spodoptera exigua nucleopolyhedrovirus (SeNPV) has been successfully applied as a bioinsecticide against S. exigua, one of the most devastating pests worldwide. However, due to limited information, the molecular mechanisms underlying interactions between S. exigua and SeNPV remain to be elucidated. In this study, RNA-Seq and differentially expressed gene (DEG) analysis of the S. exigua larva midgut were performed to explore molecular responses to SeNPV infection. A total of 1785 DEGs, including 935 upregulated and 850 downregulated genes, were identified in the midgut of SeNPV-infected S. exigua larvae. Ultrastructural observations showed that after SeNPV infection, the peritrophic matrix (PM) became a loose and highly porous surface with many clear ruptures; these changes were most likely associated with upregulation of chitin deacetylases. In addition, 124 putative innate immunity-related DEGs were identified and divided into several groups, including pattern recognition proteins, signaling pathways, signal modulation, antimicrobial peptides and detoxification. Interestingly, upregulation of some pattern recognition proteins, induction of the JAK/STAT signaling pathway and promotion of REPAT synthesis might be the main innate immunity responses occurring in the S. exigua larva midgut after SeNPV infection. According to quantitative real-time PCR, the expression profiles of 19 random DEGs were consistent with those obtained by RNA-Seq. These findings provide important basic information for understanding the molecular mechanisms of SeNPV invasion and the anti-SeNPV responses of the S. exigua midgut, promoting the utility of SeNPV as a bioinsecticide for the effective control of S. exigua and related pests.

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