A Double Mutation in the Conserved Motifs of the Helper Component Protease of Papaya Leaf Distortion Mosaic Virus for the Generation of a Cross-Protective Attenuated Strain
文献类型： 外文期刊
作者： Tuo, Decai 1 ; Zhou, Peng 1 ; Zhao, Guangyuan 1 ; Yan, Pu 1 ; Tan, Dong 1 ; Li, Xiaoying 1 ; Shen, Wentao 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
3.Hainan Key Lab Trop Microbe Resources, Haikou 571101, Hainan, Peoples R China
4.Hainan Univ, Inst Trop Agr & Forestry, Haikou 570228, Hainan, Peoples R China
关键词： attenuated strain; cross-protection; HC-Pro; papaya; PLDMV; virology
期刊名称：PHYTOPATHOLOGY （ 影响因子：4.025； 五年影响因子：4.394 ）
ISSN： 0031-949X
年卷期： 2020 年 110 卷 1 期
页码：
收录情况： SCI
摘要： Potyviral helper component protease (HC-Pro), as a major determinant of symptom expression in susceptible plants, is a likely target candidate in the production of attenuated strains for cross-protection. In this study, single or double mutations of Lys (K) to Glu (E) in the Lys-Ile-Thr-Cys motif and Arg (R) to Ile (I) in the Phe-Arg-Asn-Lys motif of the HC-Pro from the severe papaya leaf distortion mosaic virus strain DF (PLDMV-DF) reduced symptom expression and virus accumulation in infected papaya (Carica papaya) plants. The papaya plants infected with the attenuated double mutant of PLDMV-EI presented as symptomless. PLDMV-EI provided effective protection against PLDMV-DF infection in three papaya cultivars and had no effect on plant growth and development. Our result showed that PLDMV-EI is a promising mild strain for the practical use of cross-protection in the field.
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