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Cold-inducible expression of an Arabidopsis thaliana AP2 transcription factor gene, AtCRAP2, promotes flowering under unsuitable low-temperatures in chrysanthemum

文献类型： 外文期刊

作者： Luo, Chang 1 ; Liu, Hua 1 ; Ren, Junan 2 ; Chen, Dongliang 1 ; Cheng, Xi 1 ; Sun, Wei 3 ; Hong, Bo 4 ; Huang, Conglin 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing Engn Res Ctr Funct Floriculture, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing 100097, Peoples R China

2.Beijing Ind Technol Res Inst, Beijing 101111, Peoples R China

3.Capital Normal Univ, Yuquan Sch, Beijing 100195, Peoples R China

4.China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China

关键词： Chrysanthemum; DREB1A; Flowering; Low temperature; Short-day plant; SOC1

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 146 卷

页码：

收录情况： SCI

摘要： Flowering time is regulated by biotic and abiotic stresses and affected by the ambient temperature. For chrysanthemum, a low ambient growth temperature can cause a flowering delay, which limits the annual commercial production. Therefore, it is important to improve the low-temperature flowering capability of chrysanthemum through genetic modifications. Here, we isolated a natural variation of a CRT/DRE-binding factor (CBF/DREB) 3 gene, CRAP2, from the Arabidopsis thaliana accession Condara (190AV) that encodes a stop codon at position 151 of the CBF3 protein. Unlike AtCBF3, the overexpression AtCRAP2 in Arabidopsis did not cause detectable growth retardation nor delayed flowering and it conferred cold tolerance. The cold-inducible expression of AtCRAP2 in chrysanthemum promoted flowering under short-day conditions with a low 15 degrees C nighttime temperature. RNA-sequencing of rd29A:AtCRAP2 and qRT-PCR assays of flowering time-related genes and AtCRAP2 expressed at an ambient temperature revealed that AtCRAP2 positively affected SOC1 and FTL3, thereby promoting flowering under low temperature stress and short-day conditions. These results indicate that DREB genes can be used in the genetic engineering of crop plants without accompanying negative effects by modifying the encoded proteins' C termini.

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