Dramatically Enhanced lmmunochromatographic Assay Using Cascade Signal Amplification for Ultrasensitive Detection of Escherichia coil O157:K7 in Milk
文献类型： 外文期刊
作者： Fu, Jinmei 1 ; Zhou, Yaofeng 1 ; Huang, Xiaolin 1 ; Zhang, Wenjing 1 ; Wu, Yuhao 1 ; Fang, Hao 1 ; Zhang, Cunzheng 2 ; Xi 1 ;
作者机构： 1.Nanchang Univ, Nanchang, Jiangxi, Peoples R China
2.Jiangsu Acad Agr Sci, Nanjing, Peoples R China
关键词： immunochromatographic assay; cascade signal amplification; in situ gold growth; nanozyme-mediated catalytic deposition; Escherichia coli O157:H7
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2020 年 68 卷 4 期
页码：
收录情况： SCI
摘要： The conventional colloidal gold immunochromatographic assay (AuNP-ICA) cannot meet the requirements for the rapid and sensitive detection of Escherichia coli (E. coli) O157:H7 because of its poor sensitivity. Herein, a novel two-step cascade signal amplification strategy that integrates in situ gold growth and nanozyme-mediated catalytic deposition was proposed to enhance the detection sensitivity of conventional AuNP-ICA dramatically. The enhanced strip displayed ultrahigh sensitivity in E. coli O157:H7 detection and had a detection limit of 1.25 x 10(1) CFU/mL, which is approximately 400-fold lower than that of traditional AuNP-ICA (5 x 10(3) CFU/mL). The amplified strip had no background signal in the T-line zone in the absence of E. coli O157:H7 even after one round of cascade signal amplification. The enhanced strip demonstrated excellent selectivity against E. coli O157:H7 with a negligible cross-reaction to nine other common pathogens. Intra-assays and interassays showed that the improved strip has acceptable accuracy and precision for determining E. coli O157:H7. The average recoveries in a real milk sample ranged from 87.33 to 112.15%, and the coefficients of variation were less than 10%. The enhanced strip also showed great potential in detecting a single E. coli O157:H7 cell in a 75 pL solution.
- 相关文献
作者其他论文 更多>>
-
Fluorescent and Colorimetric Dual-Readout Immunochromatographic Assay for the Detection of Phenamacril Residues in Agricultural Products
作者：Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Liu, Pengyan;Jiang, Lan;Wang, Yulong;Ye, Yuhui;Zhang, Cunzheng;Jiang, Lan;Zhang, Cunzheng;Zhao, Yun;Xue, Feng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：fluorescent and colorimetric dual-readout; immunochromatographicassay; phenamacril; monoclonal antibody; polydopamine coated gold nanoparticles; quantum dots
-
Preparation of monoclonal antibody and establishment of indirect competitive chemiluminescence enzyme immunoassay for sodium pentachlorophenolate
作者：Dong, Sa;Shi, Qiuyun;Guan, Lingjun;Feng, Jianguo;Guan, Lingjun;Wang, Yulong;Liu, Pengyan;Zhang, Cunzheng
关键词：Sodium pentachlorophenate; Monoclonal antibody; ic-CLEIA; Residual detection; Animal tissues
-
Antibiotics and antibiotic resistance change bacterial community compositions in marine sediments
作者：Kong, Ming;Wang, Wanzhong;Zhang, Yu;Ma, Yan;Fang, Hao;Zhang, Shuai;Shi, Gaoling;Yan, Yan
关键词：Antibiotics; Antibiotic -resistant bacteria; Extracellular antibiotic -resistant genes; Bacterial communities; Marine sediments
-
Colorimetric and Reverse Fluorescence Dual-Signal Readout Immunochromatographic Assay for the Sensitive Determination of Sibutramine
作者：Gui, Yun;Zhang, Cunzheng;Gui, Yun;Zhao, Yun;Liu, Pengyan;Wang, Yulong;Mao, Xinxin;Zhang, Cunzheng;Zhao, Yun;Peng, Chifang;Mao, Xinxin;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：
-
Competitive ratiometric fluorescent lateral flow immunoassay based on dual emission signal for sensitive detection of chlorothalonil
作者：Fang, Xuechen;Liu, Tingting;Xue, Guangjian;Wu, Mengyun;Lai, Weihua;Peng, Juan;Fang, Xuechen;Liu, Pengyan;Zhang, Cunzheng;Xue, Chang;Zhang, Cunzheng;Hammock, Bruce D.;Hammock, Bruce D.
关键词：Fluorescent magnetic nanobeads; Aggregation-induced emission; Immunomagnetic separation; Lateral flow immunoassay; Ratiometric fluorescence
-
Amended soils with weathered coal exhibited greater resistance to aggregate breakdown than those with biochar: From the viewpoint of soil internal forces
作者：Wang, Ke;Zhang, Xiaoyuan;Zhang, Wenjing;Yang, Zhiping;Zhang, Qiang;Wang, Ke;Zhang, Wenjing;Yang, Zhiping;Zhang, Qiang;Wang, Ke;Zhang, Xiaoyuan;Zheng, Jiyong;Zhang, Xiaoyuan;Zheng, Jiyong;Cai, Jinjun;Wang, Xiaolin
关键词：Aggregate stability; Rainfall splash erosion; Application rate; Exogenous organic material; Field experiment; Soil internal forces
-
Cinnamaldehyde Inhibits Postharvest Gray Mold on Pepper Fruits via Inhibiting Fungal Growth and Triggering Fruit Defense
作者：Yang, Lifei;Liu, Xiaoli;Liu, Xiaoli;Lu, Haiyan;Zhang, Cunzheng;Chen, Jian;Shi, Zhiqi
关键词：antifungal; Botrytis cinerea; cinnamaldehyde; gray mold; induced defense; pepper fruit
