文献类型： 外文期刊

作者： Yun, Shiyu ¹ ; Guo, Yurong ¹ ; Yang, Lan ¹ ; Zhang, Xin ² ; Shen, Weiqi ¹ ; Wang, Zhuo ¹ ; Wen, Shuang ¹ ; Zhao, Dongdong ¹ ;

作者机构： 1.Shanxi Agr Univ, Coll Anim Sci & Vet Med, Taigu 030801, Peoples R China

2.Shanxi Acad Agr Sci, Inst Ind Crops, Taiyuan 030000, Shanxi, Peoples R China

关键词： Florfenicol; Mouse; Mucosal barrier; Intestinal microbiota

期刊名称：ARCHIVES OF MICROBIOLOGY （ 影响因子：2.552； 五年影响因子：2.475 ）

ISSN： 0302-8933

年卷期： 2020 年 202 卷 1 期

页码：

收录情况： SCI

摘要： Many kinds of antibiotics have effects on intestinal structure and function. In the current experimental study, we evaluate the effect of oral florfenicol on intestinal barrier in mice. Thirty adult male mice were randomly divided into two groups, the group none (N) and the group florfenicol (F), the mice in group F were orally administered florfenicol 100 mg/kg body weight (BW) for 7 days. At day 8, mice were euthanized and sampled for the analysis of alterations in genes and proteins from jejunum, jejunum morphology and microbiota analysis. Administration of florfenicol caused higher liver index (P < 0.05). In the jejunum, mucosa injury and villus rupture, compared with the group N, the villus length and V/C (villus length/crypt depth) in group F were marked decrease (P < 0.01). The transcription level of Muc2 and occludin in group F were significantly lower than those in group N (P < 0.01 or P < 0.05). The expression of APRIL, IL-17, IL-22, BAFF and sIgA on protein level were significantly down-regulated (P < 0.01 or P < 0.05), while the expression of IL-10, TGF-beta, IL-6, IL-4 were significantly up-regulated (P < 0.01) in group F. The abundances of bacteria in Firmicutes and Lactobacillus decreased significantly (P < 0.01) in group F. Our results indicated that oral administration of florfenicol might have a negative impact on functions of intestinal mucosal barrier, immune system and the intestinal microbiota.