文献类型： 外文期刊

作者： Zhang, Xin ¹ ; Bao, Yalin ¹ ; Shan, Deqi ¹ ; Wang, Zhihui ¹ ; Song, Xiaoning ¹ ; Wang, Zhaoyun ¹ ; Wang, Jiansheng ¹ ; He ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China

3.Shanxi Acad Agr Sci, Inst Ind Crops, Taiyuan 030000, Shanxi, Peoples R China

4.Nanjing Agr Univ, Cytogenet Inst, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China

5.Zhejiang Univ, Coll Agr & Biotechnol, Dept Agron, Hangzhou 310058, Zhejiang, Peoples R China

6.Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA

期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

ISSN： 0032-0889

年卷期： 2018 年 177 卷 1 期

页码：

收录情况： SCI

摘要： MicroRNAs play crucial roles in plant responses to pathogen infections. The rice blast disease, caused by the fungus Magnaporthe oryzae, is the most important disease of rice (Oryza sativa). To explore the microRNA species that participate in rice immunity against the rice blast disease, we compared the expression of small RNAs between mock-and M. oryzae-treated rice. We found that infection by M. oryzae strain Guy11 specifically induced the expression of rice miR319 and, consequently, suppressed its target gene TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTOR1 (OsTCP21), which encodes a transcription factor. Using transgenic rice that overexpresses miR319b (OE) or expresses OsTCP21-Res (which is resistant to miR319-mediated silencing), we found that OsTCP21 is a positive regulator of the rice defense response against the blast disease. When wild-type and miR319b-OE rice were infected by Guy11, multiple jasmonic acid (JA) synthetic and signaling components were suppressed, indicating that Guy11 suppresses JA signaling through inducing miR319. In particular, we found that LIPOXYGENASE2 (LOX2) and LOX5 were specifically suppressed by miR319 overexpression or by Guy11 infection. LOXs are the key enzymes of JA synthesis, which catalyze the conversion of a-linoleic acid to hydroperoxy-octadecadienoic acid. The application of a-linoleic acid rescued disease symptoms on the OsTCP21-Res rice but not wild-type rice, supporting our hypothesis that OsLOX2 and OsLOX5 are the key JA synthesis genes hijacked by Guy11 to subvert host immunity and facilitate pathogenicity. We propose that induced expression of OsLOX2/5 may improve resistance to the rice blast disease.