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Exploration of the death causes in zoea stages of Macrobrachium rosenbergii based on microbial diversity analysis

文献类型： 外文期刊

作者： Ma, Rongrong 1 ; Wang, Yuan 1 ; Zhao, Liujie 1 ; Zhou, Junfang 1 ; Zhao, Shu 1 ; Li, Xincang 1 ; Dai, Xilin 2 ; Fang, Wen 1 ;

作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Key Lab East China Sea Fishery Resources Exploita, Minist Agr, 300 Jungong Rd, Shanghai 200090, Peoples R China

2.Shanghai Ocean Univ, Coll Aquat & Life, Shanghai 201306, Peoples R China

3.Ningbo Univ, Coll Marine Sci, Ningbo 315211, Peoples R China

关键词： Macrobrachium rosenbergii; Bacterial diseases; Antibiotic and disinfectant treatment; Microbial diversity

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2020 年 519 卷

页码：

收录情况： SCI

摘要： To investigate the cause of low survival rates of Macrobrachium rosenbergii zoea in a breeding farm, traditional bacterial isolation, culture, and infection experiments were carried out. Survival rates were determined after antibacterial (oxytetracycline) bathing and disinfectant (formalin and allicin) treatments. The microbiota alterations of M. rosenbergii zoea after antibacterial bathing (zoea antibiotic treatment group and berried female antibiotic treatment group) and disinfectant treatments were identified. The results showed the presence of 42 strains of dominant bacteria that were isolated from the zoea; four strains belonging to the genus Vibrio were randomly selected and shown to have different degrees of pathogenicity, indicating mixed infection of Vibrio pathogens in the M. rosenbergii zoea. The use of an antibacterial drug and disinfectant significantly increased survival rates, indicating that bacterial infection was the main cause of the low survival rates of the M. rosenbergii zoea in this farm. High throughput sequencing results showed a decrease of bacterial diversity of the M. rosenbergii zoea after disinfectant treatment (p <= 0.05). The relative abundances of the dominant genera ( > 5%) were as follows: Vibrio was most abundant in the control group (33.52%), whereas values were low in the drug treatment group (zoea 4.9%, berried female 4.6%) and disinfectant group ( < 0.01). Enterobacter in the corresponding groups were 25.91%, 13.48% 10.11% and 60.86%, respectively; Bacillus values were 15.93%, 38%, and 40% in the control group and antibiotic bath treatment group (zoea and berried female), respectively, and were significantly higher than that in the disinfectant group ( < 5%). Lactococcus in the corresponding groups were 4.4%, 9.22%, 10.13%, and 12.44%, respectively. LEfSe analysis also indicated that Vibrio, Bacillus, and Enterobacter abundances varied significantly among different groups. In addition, the clustering and PC0A analyses showed that oxytetracycline and disinfectants have different effects on the microflora structure of M. rosenbergii zoea.

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