Cryopreservation of kelp grouper (Epinephelus moara) embryos using non-permeating cryoprotectants
文献类型： 外文期刊
作者： Zhang, Jingjing 1 ; Tian, Yongsheng 2 ; Li, Zhentong 1 ; Wu, Yuping 2 ; Li, Ziqi 1 ; Wang, Linna 2 ; Ma, Wenhui 5 ; Zhai, 1 ;
作者机构： 1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266000, Peoples R China
4.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China
5.Ming Bo Aquat Co Ltd, Laizhou 261400, Peoples R China
关键词： Cryopreservation; Sucrose; Non-permeating; Grouper embryo
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2020 年 519 卷
页码：
收录情况： SCI
摘要： In this paper, we present a new approach to cryopreserve kelp grouper embryos using non-permeating cryoprotectants (NPC). Various cryopreservation factors (embryo container, cryoprotectant solution, embryonic stage, equilibrium time, solution concentration, and thawing temperature and time) were screened in preliminary experiments. We found that using 1 M sucrose to equilibrate for 5, 6, and 7 mins and using cryotube II as the container for embryos was most effective for the kelp grouper embryos at the 16S and TB stages. Thawing was most effective at 24 degrees C for 30s. In six of the experiments, at least four groups of embryos survived freezing and thawing. In total, 1195 viable embryos were recovered from those four experiments. The survival rate was 12.60%-31.3%, the hatching rate was 5.08%-41.28%, and maximum alive time of the thawed embryos ranged from 70 h to 9 days. These results are encouraging for future experiments on cryopreservation of kelp grouper embryos using NPCs, and our study provides new cryopreservation conditions for grouper embryos.
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